Point Accumulation and Imaging in Nanoscale Topography (PAINT) is a single-molecule
technique for super-resolution microscopy that uses exchangeable single-stranded DNA
oligos or peptide-pair (docker and imager strand/peptide) to create fluorescence blinking.
This characteristic distinguishes PAINT from other widely employed techniques such as
Stochastic Optical Reconstruction Microscopy (STORM) and Photo-Activated
Localization Microscopy (PALM). PAINT exhibits various potential advantages when
compared to STORM and PALM, such as improved localization precision and increased
multiplexing capability. Furthermore, Peptide-PAINT has several other advantages. The
introduction of the docker peptide into the protein of interest, as opposed to the normal
procedure of externally introducing it in DNA-PAINT, enables the attainment of
comparable spatial resolution while streamlining experimental protocols and facilitating
its utilization in living cellular systems. Deciphering the ultrastructure details of various
cellular organelles in fixed mammalian cells using Peptide-PAINT will be discussed.
Rhabdomyosarcoma (RMS) is classified as a group of malignant neoplasms originating
from soft tissues and is recognized as one of the prevalent malignancies affecting the
pediatric and young adult populations. The aggressive subtype known as alveolar
rhabdomyosarcoma (aRMS) has been observed to exhibit a correlation with the presence
of a fusion gene called PAX3-FOXO1 or PAX7-FOXO, with PAX3-FOXO1 being more
prevalent. Both PAX3 and FOXO1 are transcription factors (TFs) that play crucial roles in
various biological processes. Nevertheless, PAX3-FOXO1 also functions as a
transcription factor (TF); however, it leads to abnormal control of transcription, hence
promoting the development of cancer. The role of multivalent interaction between the low
complexity domains (LCDs) of PAX3FOXO1 in forming transcriptional hubs, and the
underlying mechanism for transcription dysregulation will be discussed.