Fungal spores constitutes significant portion of bio aerosols commonly detected in ambient air and potentially affects human health, vegetation, and climate. Understanding the release of fungal spores is important from the perspective of prediction of their transport (by dispersion) and subsequent exposure to different receptors. The presence of large municipal waste dumpsites in many Indian cities may act as a rich source for fungal spores to sustain growth and possible release. In this presentation, the primary focus is on the identification of naturally colonized foodborne fungi along with their spore release rate. To do this, the most dominant naturally colonized foodborne fungal species are identified along with their substrate specificity using Next Generation Sequencing (NGS) technique and found that very limited fungal communities are associated with each kind of food substrate. The diversity of colonized internal fungal communities that were present in the food substrates were also identified and compared with the diversity of fungal species in extracts of food substrates to evaluate the contribution of inherent fungal species in the food substrates to the emission of fungal spores in the environment. From the colonized fungi on food substrates, the magnitude of fungal spore production and their composite spore emission flux was measured to extract the energy required to detach and aerosolize a single spore from the conidiophore using the developed mathematical model. Further, the effect of relative humidity cycling on fungal spore emission flux has shown that the fungal spore moisture drying is instantaneous which enhances the release rate during the initial air exposure.