Cell culture is a random phenomenon with no control over its spatial and temporal aspects as well as distribution of population density. This makes operations such as live cell tracing, isolation, targeted stimulation and delivery for downstream analysis very difficult. Micro electro mechanical systems (MEMS) are thus designed to control the spatial distribution of cells over the substrate in its live culture. MEMS make use of microfabrication technology along with concepts of energy, chemistry, physics and mechanics to manipulate cells with up to single cell precision. The technologies under discussion will include Micro contact printing, hydrodynamic flow confinement, bio printing, droplet microfluidics, micro pipette array, optical trapping, acoustic tweezers and dielectrophoretic technique. These techniques are cell manipulators which can give unique cell patterned substrates for better accessibility to investigate individual cells and their inter-cellular interactions. The cell patterning gives high spatial resolution without unnecessary inference from unwanted chemicals. The microfabrication processes involved in each of the techniques will also be discussed to understand the complexities involved in fabrication of these technologies. The study of such tools brings an ease in the process of development of therapeutic and diagnostic tools. This technology can especially be beneficial in the research of regenerative medicine and tissue engineering. The increase in ease and accessibility to technology used for the study of individual cells can revolutionize personalized medicine.