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Microfluidic devices for the synthesis of anisotropic gold nanostructures in cancer cell therapy and diagnosis

Microfluidic devices for the synthesis of anisotropic gold nanostructures in cancer cell therapy and diagnosis

Date5th Mar 2021

Time03:00 PM

Venue https://meet.google.com/uoy-xqdc-ryz

PAST EVENT

Details

Cancer is the second leading cause of death globally; its early detection and diagnosis can significantly reduce the mortality rate. Cancer cell therapy and its diagnosis can be achieved by introducing exogenous molecules such as drugs, genes or proteins into the cancer cells. Currently, three approaches are available for molecular delivery; viral, chemical and physical. Compared to viral and chemical methods, physical approaches possess several advantages, specifically the light energy-based method, known as photoporation. It uses high-intensity light pulses to generate cell membrane pores, through which exogenous molecules can enter easily. By incorporating anisotropic gold nanomaterials, photoporation can be achieved at low-intensity light pulses. Using the conventional batch reactor method, it isn't easy to attain a controlled synthesis of nanomaterials with high reproducibility and monodispersity. In addition, exogenous molecular delivery using nanomaterials demands large production of nanocarriers, which is not possible using a conventional batch reactor due to poor mixing of different phases during synthesis and variation in reaction conditions. Therefore, I am proposing various microfluidic geometries for the controlled synthesis of anisotropic gold nanomaterials and their usage in intracellular delivery of exogenous molecules using laser pulses. Compared to spherical gold nanomaterials, anisotropic nanostructures have plasmonic peak located at the near-infrared region, which is beneficial for photothermal applications. The device can be further modified to simultaneously synthesise the anisotropic gold nanostructures and deliver drugs at the intracellular level using photoporation, enhancing the cellular transfection efficiency and cell viability.

Speakers

Ms. I Kavitha, ED18D600

Department of Engineering Design