Single cell technology provides a high-performance platform for understanding cell heterogeneity, basic cellular functions, cell to cell interactions, neuronal network formation, drug screening and so on. The basic step involved in single cell technology is the precise positioning or patterning of cells into spatially distinct areas by regulating cell adhesion and retaining them in the position. Precise spatial positioning of the cells can be done by any physical trapping method or by modifying substrate through physical or chemical means. In the present study we are demonstrating a method for single cell patterning by surface modification of the substrate and optoporation based cellular delivery on a monolayer of patterned cells. Cell patterning is done on glass substrates having rGO areas and non-rGO areas. Nanoscale textured rGO substrate can provide contact guidance for aligning cells over rGO areas compared to rest smooth non rGO areas. Considering high photothermal conductivity of reduced graphene oxide(rGO) we have fabricated a device for optoporation based applications. The device consists of a rectangular array of square shaped rGO regions on a glass surface. The 2D drawings of the device were developed using AutoCAD software. The devices with rectangular array of graphene oxide (GO) squares were fabricated using photolithography-liftoff method followed by reduction of these GO areas to rGO areas using a high-power UV source. This device can be used for single optoporation studies on a patterned monolayer of cells using laser scanning.