Enzyme electrocatalysis and enzymatic fuel cells hold great promise for sustainable energy conversion. Enzymes, as biocatalysts, possess remarkable qualities such as high catalytic efficiency, substrate specificity, and selectivity. In this presentation, we will delve into two bioengineering studies that explored the integration of pyrene-stacked C-nanostructures and Buckypaper-based biocathodes into enzymatic fuel cells to enhance their overall performance. Our discussion encompasses various topics, including enzyme immobilization techniques, electron transfer mechanisms, and system optimization. It is important to note that enzyme electrocatalysis and enzymatic fuel cells have broader applications beyond energy technologies, including clean synthesis platforms for fine and precious chemicals, sensors, and biomedical devices. These advancements offer exciting avenues for sustainable and versatile technologies in various fields.