The two major steps involved in traffic signal design are the choice of phase sequence and the allocation of green times to each phase. The former can be done relying on two distinct approaches – non-overlap (phase based) approach and Overlap (Group-based) approach. The non-overlap approach though simple and more widely used, lacks the flexibility to adapt to varying traffic demand. Overlap (group-based) signal timing has been gaining traction worldwide for the last few decades due to its versatility in accommodating varying traffic demands. Studies reported in the literature have compared the operational performance of these approaches theoretically and have shown that overlap based phasing strategy performs better than non-overlap based phasing strategy. These studies mostly presented a theoretical comparison of the two approaches without comparing the operational performance under different traffic scenarios in real–field conditions. Further, the performance of an overlap signal timing approach could be impacted by the presence or absence of dedicated turn lanes and the extent of lane-discipline in the traffic stream. There are no studies which compared the operational performance of the overlap-based and non-overlap-based timing strategies under Heterogeneous Less-Lane Disciplined (HLLD) traffic conditions. The current study quantitatively compares these two phasing strategies under both Homogeneous Lane Disciplined (HoLD) and HLLD traffic conditions for varying traffic conditions. Experimental results show that the overlap-based method has maximum operational benefits when the traffic demand and the skewness of traffic demand are high. Up to 58% and 75% reduction in average delay for fixed-time control and actuated-control respectively are achieved using the overlap-based phasing strategy for a scenario with an intersection degree of saturation of 0.9 and a split ratio (skewness of demand) of 0.5. Further, the overlap-based approach was observed to be less efficient under HLLD traffic conditions due to lack of lane discipline with a 29% average delay reduction compared to a 58% reduction under HoLD traffic conditions