HOMODIMER DATA
The binding affinity (dissociation constants) and binding free energy (ΔΔG) values are provided below for homodimer complexes.
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| Protein | UniProt | PDB | Mutation | Technique | Experiment conditions | Temperature | pH | Wild-type Dissociation constant (M) | Mutant Dissociation constant (M) | ΔΔG in kcal/mol | Pubmed/Reference | Exact location | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A43 | N325A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate buffer | 296 | 7.3 | 8.33e-06 | 7.35e-06 | -0.07 | 12761222 | p.27926, Table 1 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A43 | V323A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate, 150 mM NaCl buffer | 296 | 7.3 | 6.94e-06 | 1e-05 | 0.21 | 12761222 | p.27926, Table 1 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A43 | D284A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate buffer | 296 | 7.3 | 8.33e-06 | 1.27e-05 | 0.25 | 12761222 | p.27926, Table 1 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A43 | T280A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate buffer | 296 | 7.3 | 8.33e-06 | 1.52e-05 | 0.35 | 12761222 | p.27926, Table 1 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | Q324A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate, 150 mM NaCl buffer | 296 | 7.3 | 6.94e-06 | 3.32e-07 | -1.79 | 15588834 | p.899, Table 4 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | E312K | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate buffer | 296 | 7.3 | 8.33e-06 | 7.990000000000001e-07 | -1.38 | 15588834 | p.895, Table 1 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | E319A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate, 150 mM NaCl buffer | 296 | 7.3 | 6.94e-06 | 1.88e-06 | -0.77 | 15588834 | p.899, Table 4 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | E312A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate, 150 mM NaCl buffer | 296 | 7.3 | 6.94e-06 | 2.27e-06 | -0.66 | 15588834 | p.899, Table 4 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | S310A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate, 150 mM NaCl buffer | 296 | 7.3 | 6.94e-06 | 3.09e-06 | -0.48 | 15588834 | p.899, Table 4 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | R286M | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate buffer | 296 | 7.3 | 8.33e-06 | 1.3699999999999998e-05 | 0.29 | 15588834 | p.895, Table 1 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | K331A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate buffer | 296 | 7.3 | 8.33e-06 | 1.9600000000000002e-05 | 0.5 | 15588834 | p.895, Table 1 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | R286E | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate buffer | 296 | 7.3 | 8.33e-06 | 4.55e-05 | 1.0 | 15588834 | p.895, Table 1 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | K331E | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate buffer | 296 | 7.3 | 8.33e-06 | 4.7600000000000005e-05 | 1.03 | 15588834 | p.895, Table 1 | |
| HIV-1 capsid protein C-terminal domain (CA-C) | P12497 | 1A8O | R286A | Analytical gel-filtration, frontal elution | 15 ml aliquots of CA-C samples with total monomer concentration from 0.5 to 60 μM serially applied to analytical Superdex 75 column (Amersham Biotech); 25mM sodium phosphate buffer | 296 | 7.3 | 8.33e-06 | 2.5 | 7.42 | 15588834 | p.895, Table 1 | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | W77R | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 2.66e-06 | -0.36 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | A50S | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 4.33e-06 | -0.08 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | D49A | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 4.33e-06 | -0.08 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | R164A | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 1.09e-05 | 0.46 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | A50T | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 1.21e-05 | 0.52 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | R56K | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 1.67e-05 | 0.71 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | W77G | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 7.41e-05 | 1.58 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | R56E | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 0.00013000000000000002 | 1.91 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | D49R | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 0.00023799999999999998 | 2.26 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | R56D | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 0.00027400000000000005 | 2.34 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | R56M | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 0.00049 | 2.68 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | S166P | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 0.0005679999999999999 | 2.76 | 12006601 | p.25737, Table I | |
| Bovine β-lactoglobulin (β-LG) | P02754 | 1BEB | H162P | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge; 20 Mm sodium phosphate buffer containing 20 Mm NaCl | 293 | 6.5 | 4.93e-06 | 0.00119 | 3.19 | 12006601 | p.25737, Table I | |
| Glutathione S-transferase, isozyme A1-1 (GST A1-1) | P08263 | 1GUH | F136Q | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge with An-60 Ti rotor at 15k, 17k, and 20k rpm; 0.1 M KPO4 and 1 mM EDTA | 283 | 6.5 | 3.4e-07 | 8e-08 | -0.81 | 15035604 | p.3332, Table 5 | wildtype data obtained for GST with His tag |
| Glutathione S-transferase, isozyme A1-1 (GST A1-1) | P08263 | 1GUH | R69Q | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge with An-60 Ti rotor at 15k, 17k, and 20k rpm; 0.1 M KPO4 and 1 mM EDTA | 283 | 6.5 | 3.4e-07 | 9.2e-07 | 0.56 | 15035604 | p.3332, Table 5 | with Histidine tag; wildtype data obtained for GST with His tag |
| Glutathione S-transferase, isozyme A1-1 (GST A1-1) | P08263 | 1GUH | F52A | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge with An-60 Ti rotor at 15k, 17k, and 20k rpm; 0.1 M KPO4 and 1 mM EDTA | 283 | 6.5 | 3.4e-07 | 3.1e-06 | 1.24 | 15035604 | p.3332, Table 5 | wildtype data obtained for GST with His tag |
| Glutathione S-transferase, isozyme A1-1 (GST A1-1) | P08263 | 1GUH | R69E | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge with An-60 Ti rotor at 15k, 17k, and 20k rpm; 0.1 M KPO4 and 1 mM EDTA | 283 | 6.5 | 3.4e-07 | 3.4e-06 | 1.29 | 15035604 | p.3332, Table 5 | with Histidine tag; wildtype data obtained for GST with His tag |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | N140D | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.00398 | -0.37 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | D77V | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.00589 | -0.14 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | D77Y | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.00603 | -0.12 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | D91A | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.007240000000000001 | -0.01 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | D102N | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.00813 | 0.05 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | N140K | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.01 | 0.18 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | D91V | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.0158 | 0.45 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | N87S | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.0178 | 0.52 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | D102G | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.0229 | 0.67 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | N87K | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.0263 | 0.75 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | S106L | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.0282 | 0.79 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | L145S | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.0324 | 0.87 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | G115A | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.0363 | 0.94 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | L39V | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.0398 | 1.0 | 20189984 | p.19550, Table 1 | |
| Superoxide dismutase (SOD1) | P00441 | 1HL5 | N87D | Stopped-flow spectrofluorometry | X-17MV stopped-flow spectrofluorometer (Applied Photophysics, Leatherhead, UK); standard buffer 10 Mm MES, 10 Mm EDTA, 5.8 M urea | 298 | 6.3 | 0.007409999999999999 | 0.0398 | 1.0 | 20189984 | p.19550, Table 1 | |
| Human thymidylate synthase (hTS) | P04818 | 1HVY | K47A | Förster resonance energy transfer | Fluorescence spectra measured on a Spex-Jobin Yvon Fluoromax2 spectrofluorometer; UV-visible absorption spectra measured with a Varian Cary 100 spectrophotometer | 299 | 5e-08 | 6e-07 | 1.48 | 25798950 | p.3573, Table 1 (values were converted to kcal/mol) | ||
| Human thymidylate synthase (hTS) | P04818 | 1HVY | Y202A | Förster resonance energy transfer | Fluorescence spectra measured on a Spex-Jobin Yvon Fluoromax2 spectrofluorometer; UV-visible absorption spectra measured with a Varian Cary 100 spectrophotometer | 299 | 5e-08 | 9e-07 | 1.72 | 25798950 | p.3573, Table 1 (values were converted to kcal/mol) | ||
| Human thymidylate synthase (hTS) | P04818 | 1HVY | L198A | Förster resonance energy transfer | Fluorescence spectra measured on a Spex-Jobin Yvon Fluoromax2 spectrofluorometer; UV-visible absorption spectra measured with a Varian Cary 100 spectrophotometer | 299 | 5e-08 | 3.7e-06 | 2.56 | 25798950 | p.3573, Table 1 (values were converted to kcal/mol) | ||
| Human thymidylate synthase (hTS) | P04818 | 1HVY | W182A | Förster resonance energy transfer | Fluorescence spectra measured on a Spex-Jobin Yvon Fluoromax2 spectrofluorometer; UV-visible absorption spectra measured with a Varian Cary 100 spectrophotometer | 299 | 5e-08 | 4.7e-06 | 2.7 | 25798950 | p.3573, Table 1 (values were converted to kcal/mol) | ||
| Human thymidylate synthase (hTS) | P04818 | 1HVY | I178A | Förster resonance energy transfer | Fluorescence spectra measured on a Spex-Jobin Yvon Fluoromax2 spectrofluorometer; UV-visible absorption spectra measured with a Varian Cary 100 spectrophotometer | 299 | 5e-08 | 1.2e-05 | 3.26 | 25798950 | p.3573, Table 1 (values were converted to kcal/mol) | ||
| Human thymidylate synthase (hTS) | P04818 | 1HVY | F59A | Förster resonance energy transfer | Fluorescence spectra measured on a Spex-Jobin Yvon Fluoromax2 spectrofluorometer; UV-visible absorption spectra measured with a Varian Cary 100 spectrophotometer | 299 | 5e-08 | 0.00013000000000000002 | 4.67 | 25798950 | p.3573, Table 1 (values were converted to kcal/mol) | ||
| Fibroblast growth factor (FGF9) | P31371 | 1IHK | N143T | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Coulter XL-I analytical ultracentrifuge; 25 mM ammonium acetate buffer, 120 mM NaCl; data obtained at 12, 14 and 16k rpm for FGF9-WT and at 14, 16 and 18k rpm for FGF9-mutant; Protein concentrations were 0.4, 0.3 or 0.2 mg/ml. | 293 | 5.5 | 9.619999999999999e-08 | 8.4e-06 | 2.6 | 19219044 | p.291, para 4 | |
| Nuclear transport factor 2 (NTF2) | P61972 | 1JB2 | M84E | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge with An-60 Ti rotor | 278 | 1.1e-06 | 5e-06 | 0.84 | 11846560 | pp.467, para 2 | ||
| Nuclear transport factor 2 (NTF2) | P61972 | 1JB4 | M102E | Sedimentation Equilibrium Analytical Ultracentrifugation | Beckman Optima XL-A analytical ultracentrifuge with An-60 Ti rotor | 278 | 1.1e-06 | 2e-05 | 1.6 | 11846560 | pp.467, para 2 | ||
| SARS coronavirus 3C-like protease (SARS-CoV 3CLpro) | P0C6X7 | 1UK3 | S3384A | Sedimentation Equilibrium Analytical Ultracentrifugation | 10 mM Tris-HCl, 0.1 M NaCl, 1 mM TCEP, and 1 mM EDTA; Beckman-Coulter XL-I analytical ultracentrifuge with An60Ti rotor at 50000 rpm; protein concentrations used - 0.1 mg/mL (2.9 μM), 0.25 mg/mL (7.4 μM), 0.5 mg/mL (14.8 μM), 0.75 mg/mL (22.1 μM), 1 mg/mL (29.5 μM), and 2 mg/mL (59.1 μM) | 293 | 7.5 | 1.3e-06 | 3.3e-06 | 0.54 | 17154528 | p. 14912, Table 1 | |
| SARS coronavirus 3C-like protease (SARS-CoV 3CLpro) | P0C6X7 | 1UK3 | S3379A | Sedimentation Equilibrium Analytical Ultracentrifugation | 10 mM Tris-HCl, 0.1 M NaCl, 1 mM TCEP, and 1 mM EDTA; Beckman-Coulter XL-I analytical ultracentrifuge with An60Ti rotor at 50000 rpm; protein concentrations used - 0.1 mg/mL (2.9 μM), 0.25 mg/mL (7.4 μM), 0.5 mg/mL (14.8 μM), 0.75 mg/mL (22.1 μM), 1 mg/mL (29.5 μM), and 2 mg/mL (59.1 μM) | 293 | 7.5 | 1.3e-06 | 1.29e-05 | 1.34 | 17154528 | p. 14912, Table 1 | |
| SARS coronavirus 3C-like protease (SARS-CoV 3CLpro) | P0C6X7 | 2BX4 | N3268A | Sedimentation Equilibrium Analytical Ultracentrifugation | 10 mM Tris-HCl, 0.1 M NaCl, 1 mM TCEP, and 1 mM EDTA; Beckman-Coulter XL-I analytical ultracentrifuge with An60Ti rotor at 50000 rpm; protein concentrations used - 0.25 mg/mL (7.4 μM), 0.5 mg/mL (14.8 μM), and 1 mg/mL (29.5 μM) | 293 | 7.4 | 1.3e-06 | 2.5e-05 | 1.72 | 20420403 | p. 4312, Table 2 | |
| Bovine nuerophysin-1 (BNP-1) | P01175 | 2HNU | Q86E | NMR Spectroscopy | Varian Inova 600 MHz spectrometer; samples dissolved in D2O containing 10 mM phosphate buffer readjusted to final pH 6.2 | 298 | 6.2 | 0.00023799999999999998 | 9.520000000000001e-05 | -0.54 | 17192588 | p.54, Table 1 | |
| Bovine nuerophysin-1 (BNP-1) | P01175 | 2HNU | K90A | NMR Spectroscopy | Varian Inova 600 MHz spectrometer; samples dissolved in D2O containing 10 mM phosphate buffer readjusted to final pH 6.2 | 298 | 6.2 | 0.00023799999999999998 | 0.000143 | -0.3 | 17192588 | p.54, Table 1 | |
| Bovine nuerophysin-1 (BNP-1) | P01175 | 2HNU | Q89D | NMR Spectroscopy | Varian Inova 600 MHz spectrometer; samples dissolved in D2O containing 10 mM phosphate buffer readjusted to final pH 6.2 | 298 | 6.2 | 0.00023799999999999998 | 0.00018999999999999998 | -0.13 | 17192588 | p.54, Table 1 | |
| Bovine nuerophysin-1 (BNP-1) | P01175 | 2HNU | S87G | NMR Spectroscopy | Varian Inova 600 MHz spectrometer; samples dissolved in D2O containing 10 mM phosphate buffer readjusted to final pH 6.2 | 298 | 6.2 | 0.00023799999999999998 | 0.000229 | -0.02 | 17192588 | p.54, Table 1 | |
| Bovine nuerophysin-1 (BNP-1) | P01175 | 2HNU | S87A | NMR Spectroscopy | Varian Inova 600 MHz spectrometer; samples dissolved in D2O containing 10 mM phosphate buffer readjusted to final pH 6.2 | 298 | 6.2 | 0.00023799999999999998 | 0.000357 | 0.24 | 17192588 | p.54, Table 1 | |
| Bovine nuerophysin-1 (BNP-1) | P01175 | 2HNU | Q89A | NMR Spectroscopy | Varian Inova 600 MHz spectrometer; samples dissolved in D2O containing 10 mM phosphate buffer readjusted to final pH 6.2 | 298 | 6.2 | 0.00023799999999999998 | 0.000429 | 0.35 | 17192588 | p.54, Table 1 | |
| Bovine nuerophysin-1 (BNP-1) | P01175 | 2HNU | Q89V | NMR Spectroscopy | Varian Inova 600 MHz spectrometer; samples dissolved in D2O containing 10 mM phosphate buffer readjusted to final pH 6.2 | 298 | 6.2 | 0.00023799999999999998 | 0.000524 | 0.47 | 17192588 | p.54, Table 1; p.56, Table 2 | Dimerization is understood to mean association of the monomers; therefore, opposite sign taken for the ΔΔG values from Table 2. |
| Bovine nuerophysin-1 (BNP-1) | P01175 | 2HNU | Q86A | NMR Spectroscopy | Varian Inova 600 MHz spectrometer; samples dissolved in D2O containing 10 mM phosphate buffer readjusted to final pH 6.2 | 298 | 6.2 | 0.00023799999999999998 | 0.000595 | 0.54 | 17192588 | p.54, Table 1; p.56, Table 2 | Dimerization is understood to mean association of the monomers; therefore, opposite sign taken for the ΔΔG values from Table 2. |
| Bovine nuerophysin-1 (BNP-1) | P01175 | 2HNU | Q89G | NMR Spectroscopy | Varian Inova 600 MHz spectrometer; samples dissolved in D2O containing 10 mM phosphate buffer readjusted to final pH 6.2 | 298 | 6.2 | 0.00023799999999999998 | 0.0006190000000000001 | 0.57 | 17192588 | p.54, Table 1 | |
| Coiled-coil segment of partner and localizer of BRCA2 (PALB2cc) | Q3U0P1 | 6E4H | L24A | NMR Spectroscopy | Bruker AVANCE 600 MHz or 800 MHz NMR spectrometer; 20 mM MES, 200 mM NaCl, 10 mM DTT, 5 mM CaCl2 buffer | 293 | 6.5 | 8e-05 | 0.007 | 2.6 | 30289697 | p.6585, para 1 | |
| Bovine nuerophysin-1 (BNP-1) | P01175 | H111E | NMR Spectroscopy | 0.1 M ammonium acetate/D2O | 298 | 6.2 | 0.0001 | 0.001 | 1.36 | 10891091 | p. 8087, Table 1 | ||
| Bovine nuerophysin-1 (BNP-1) | P01175 | H111D | NMR Spectroscopy | 0.1 M ammonium acetate/D2O | 298 | 6.2 | 0.0001 | 0.0016699999999999998 | 1.67 | 10891091 | p. 8087, Table 1 | ||
| lambda cI repressor | P03034 | Y211H | Analytical gel chromatography | Sephadex G-100 column; 10mM Bis-Tris, 200 mM KC1, 2.5 mM MgCl2, 1 mM CaCl2, 100 μg/mL BSA | 293 | 7.0 | 5.560000000000001e-09 | 7.69e-09 | 0.19 | 8031775 | p.8402, Table 2 | ||
| lambda cI repressor | P03034 | E103K | Analytical gel chromatography | Sephadex G-100 column; 10mM Bis-Tris, 200 mM KC1, 2.5 mM MgCl2, 1 mM CaCl2, 100 μg/mL BSA | 293 | 7.0 | 5.560000000000001e-09 | 9.09e-09 | 0.29 | 8031775 | p.8402, Table 2 | ||
| lambda cI repressor | P03034 | G148D | Analytical gel chromatography | Sephadex G-100 column; 10mM Bis-Tris, 200 mM KC1, 2.5 mM MgCl2, 1 mM CaCl2, 100 μg/mL BSA | 293 | 7.0 | 5.560000000000001e-09 | 1.33e-08 | 0.51 | 8031775 | p.8402, Table 2 | ||
| lambda cI repressor | P03034 | K193N | Analytical gel chromatography | Sephadex G-100 column; 10mM Bis-Tris, 200 mM KC1, 2.5 mM MgCl2, 1 mM CaCl2, 100 μg/mL BSA | 293 | 7.0 | 5.560000000000001e-09 | 1.37e-08 | 0.53 | 8031775 | p.8402, Table 2 | ||
| lambda cI repressor | P03034 | S229R | Analytical gel chromatography | Sephadex G-100 column; 10mM Bis-Tris, 200 mM KC1, 2.5 mM MgCl2, 1 mM CaCl2, 100 μg/mL BSA | 293 | 7.0 | 5.560000000000001e-09 | 2.56e-07 | 2.23 | 8031775 | p.8402, Table 2 | ||
| lambda cI repressor | P03034 | S229N | Analytical gel chromatography | Sephadex G-100 column; 10mM Bis-Tris, 200 mM KC1, 2.5 mM MgCl2, 1 mM CaCl2, 100 μg/mL BSA | 293 | 7.0 | 5.560000000000001e-09 | 2.7e-06 | 3.6 | 8031775 | p.8402, Table 2 |