WILD-TYPE DATA
The binding affinity (dissociation constants) and binding free energy (ΔG) values are provided below for wild-type protein-protein complexes.
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| S.no. | PDB | Chains | Protein 1 | Protein 2 | Functional Class | Experimental Technique | Ionic Conditions | Temperature | pH | Pubmed/Location | Dissociation constant (M) | ΔG (kcal/mol) | Notes |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Anabaena sp. flavodoxin | Anabaena sp. ferredoxin-NADP+ reductase | OE | FNR-dependent NADPH cytochrome c reductase activity assayed by using Fld as the electron carrier from FNR to cytochrome c | 50 mM Tris-HCl | 298 | 8 | 15628849 (Table 2, p. 100) | 3.00E-06 | -7.53 | |||
| 2 | Anabaena sp. flavodoxin | Anabaena sp. Photosystem I | OE | FNR-dependent NADPH cytochrome c reductase activity assayed by using Fld as the electron carrier from FNR to cytochrome c | 20mM Tricine-KOH, 2mM MgCl2 | 295 | 7.5 | 15628849 (Table 2, p. 100) | 5.00E-06 | -7.16 | |||
| 3 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Stopped-flow Fluorescence | 50mM Tris-HCl | 298 | 8 | 7739054 (Table 1, p. 481) | 1.00E-14 | -15.17 | |
| 4 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Competitive binding with [3H] Barstar or Stopped-flow spectrophotometry | 100mM NaCl, 10mM acetate buffer | 298 | 4.5 | 8494892 (Table I, p. 5148) | 9.35E-11 | -13.68 | |
| 5 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Competitive binding with [3H] Barstar or Stopped-flow spectrophotometry | 100mM NaCl, 10mM acetate buffer | 298 | 5 | 8494892 (Table I, p. 5148) | 1.56E-11 | -14.74 | |
| 6 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Competitive binding with [3H] Barstar or Stopped-flow spectrophotometry | 100mM NaCl, 10mM MES | 298 | 5.5 | 8494892 (Table I, p. 5148) | 4.50E-12 | -15.47 | |
| 7 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Competitive binding with [3H] Barstar or Stopped-flow spectrophotometry | 100mM NaCl, 10mM MES | 298 | 6 | 8494892 (Table I, p. 5148) | 1.15E-12 | -16.28 | |
| 8 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Competitive binding with [3H] Barstar or Stopped-flow spectrophotometry | 100mM NaCl, 10mM MOPS | 298 | 7 | 8494892 (Table I, p. 5148) | 1.48E-13 | -17.49 | |
| 9 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Competitive binding with [3H] Barstar or Stopped-flow spectrophotometry | 100mM NaCl, 10mM Trizma base | 298 | 8 | 8494892 (Table I, p. 5148) | 1.30E-13 | -17.58 | |
| 10 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Competitive binding with [3H] Barstar or Stopped-flow spectrophotometry | 100mM NaCl, 10mM Trizma base | 298 | 9 | 8494892 (Table I, p. 5148) | 1.83E-13 | -17.37 | |
| 11 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Fluorescence | 298 | 8637875 (Table 1, p. 2346) | 6.00E-14 | -18.03 | |||
| 12 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Isothermal Titration Calorimetry | 24mM Hepes, 1mM DTT | 298 | 8 | 9126847 (Table 2, p. 699) | 1.00E-14 | -19.09 | |
| 13 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Isothermal Titration Calorimetry | 39mM Hepes, 1 mM DTT | 298 | 8 | 9126847 (Table 3, p. 700) | 1.00E-14 | -19.09 | |
| 14 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Isothermal Titration Calorimetry | 35mM Tes, 1 mM DTT | 298 | 8 | 9126847 (Table 3, p. 700) | 1.00E-14 | -19.09 | |
| 15 | 1BRS | A:D | Bacillus amyloliquefaciens barnase/ribonuclease | Bacillus amyloliquefaciens barstar | EI | Surface Plasmon Resonance | Hepes buffer with 150mM NaCl, 3mM EDTA, 0.005% Tween 20 | 298 | 7.4 | Water-mediated interaction at a protein-protein interface, Chemical Physics, Volume 307, Issues 2-3, 2004 (Table I, p. 113) | 1.32E-10 | -13.47 | |
| 16 | 1TM1 | E:I | Bacillus amyloliquefaciens subtilisin BPN'/subtilisin novo | Hordeum vulgare chymotrypsin inhibitor 2 (CI2) | EI | Stopped-flow fluorescence | 100 mM Tris-HCl | 298 | 8.6 | 10065709 (Table II, p. 44) | 1.92E-12 | -15.98 | |
| 17 | 1SBN | E:I | Bacillus amyloliquefaciens subtilisin BPN'/subtilisin novo | Hirudo medicinalis eglin c | EI | 298 | 1992167 (Table 1, p. 357) | 3.20E-11 | -14.31 | ||||
| 18 | 1TM1 | E:I | Bacillus amyloliquefaciens subtilisin BPN'/subtilisin novo | Hordeum vulgare chymotrypsin inhibitor 2 (CI2) | EI | Inhibition constant Ki calculated from the reduced rates of subtilisin hydrolysis of a chromogenic substrate | 0.1 M Tris, 1 mM DTT | 298 | 8.6 | 15504027 (Table 1, p. 13650) | 3.00E-12 | -15.71 | |
| 19 | 1TM1 | E:I | Bacillus amyloliquefaciens subtilisin BPN'/subtilisin novo | Hordeum vulgare chymotrypsin inhibitor 2 (CI2) | EI | Spectrophotometry | 298 | 7947796 (Table 3, p. 13883) | 7.00E-13 | -16.57 | |||
| 20 | 2SIC | E:I | Bacillus amyloliquefaciens subtilisin BPN'/subtilisin novo | Streptomyces albogriseolus subtilisin inhibitor | EI | Spectroscopy | 5mM phosphate buffer with 0.005% (w/v) Triton X-100 | 298 | 7 | 8276767 (Table 1, p. 555) | 1.80E-11 | -14.65 | |
| 21 | Bacillus anthracis beta-lactamase bla1 | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | Spectroscopy | 50 mm phosphate buffer with 1 mg/ml BSA | 298 | 7 | 15284234 (Table II, p. 42862) | 2.50E-09 | -11.73 | |||
| 22 | 1CSE | E:I | Bacillus licheniformis subtilisin carlsberg | Hirudo medicinalis eglin c | EI | Spectroscopy | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 9048543 (Table 1, p. 1602) | 1.12E-12 | -16.08 | |
| 23 | 1R0R | E:I | Bacillus licheniformis subtilisin carlsberg | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Spectroscopy | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 11171964 (data previously accessible online here, please check SKEMPI Notes for details on the data source) | 2.94E-11 | -14.17 | |
| 24 | 1R0R | E:I | Bacillus licheniformis subtilisin carlsberg | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Spectroscopy | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 12767228 (Tables 1 and 2, p. 6462, Table 3, p. 6463) | 2.72E-11 | -14.21 | |
| 25 | 1R0R | E:I | Bacillus licheniformis subtilisin carlsberg | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Titration | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 9047374 (Table 1, p. 443) | 2.94E-11 | -14.17 | |
| 26 | 1R0R | E:I | Bacillus licheniformis subtilisin carlsberg | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | 294 | Stephen Ming-teh Lu, PhD Thesis, Purdue University, 2000 (See SKEMPI for data source) | 2.94E-11 | -14.17 | ||||
| 27 | 2B42 | A:B | Bacillus subtilis endo-1,4-beta-xylanase A | Triticum aestivum xylanase inhibitor TAXI-I | EI | Surface Plasmon Resonance | 10mM sodium acetate | 295 | 5 | 16279951 (Table 2, p. 5876) | 1.07E-09 | -12.11 | |
| 28 | 2BTF | A:P | Bovine actin, cytoplasmic 1 | Bovine profilin-1 | High Shear Viscometry | 0.01 M imidazole, 0.15mM CaCl2, 1.5mM DTE, 1mM ATP | 298 | 7 | 9788869 (Figure 4, p. 3267) | 2.30E-06 | -7.69 | ||
| 29 | 1CBW | FGH:I | Bovine alpha-chymotrypsin/chymotrypsinogen A | Bovine pancreatic trypsin inhibitor (BPTI) | EI | Spectroscopy | 50mM Tris, 20mM CaCl2, 0.01 % (v/v) Triton X-100 | 295 | 8.3 | 10339415 (Table 1, p. 177) | 1.14E-08 | -10.72 | |
| 30 | 1T7C | A:B | Bovine alpha-chymotrypsin/chymotrypsinogen A | Bovine pancreatic trypsin inhibitor (BPTI) | EI | monitoring the release of p-nitroanilide using spectrophotometry | 50mM Tris-HCl, 20mM CaCl2, 0.005% Triton X-100 | 295 | 8.2 | 8784199 (Table 1, p. 11439) | 1.10E-08 | -10.74 | |
| 31 | 1N8O | ABC:E | Bovine alpha-chymotrypsin/chymotrypsinogen A | Escherichia coli ecotin | EI | Fluorescence | 50mM Tris-HCl, 20mM CaCl2, 0.005% (w/v) Triton X-100 | 294 | 8.3 | 8143850 (Table 1, p.59) | 4.00E-12 | -15.33 | |
| 32 | 1ACB | E:I | Bovine alpha-chymotrypsin/chymotrypsinogen A | Hirudo medicinalis eglin c | EI | Spectroscopy | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 9048543 (Table 1, p. 1602) | 1.49E-12 | -15.91 | |
| 33 | 1CHO | EFG:I | Bovine alpha-chymotrypsin/chymotrypsinogen A | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Spectroscopy | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 11171964 (data previously accessible online here, please check SKEMPI Notes for details on the data source) | 5.26E-12 | -15.17 | |
| 34 | 1CHO | EFG:I | Bovine alpha-chymotrypsin/chymotrypsinogen A | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Spectroscopy | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 12767228 (Tables 1 and 2, p. 6462, Table 3, p. 6463) | 4.83E-12 | -15.22 | |
| 35 | 1CHO | EFG:I | Bovine alpha-chymotrypsin/chymotrypsinogen A | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Titration | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 9047374 (Table 1, p. 443) | 5.26E-12 | -15.17 | |
| 36 | 1CHO | EFG:I | Bovine alpha-chymotrypsin/chymotrypsinogen A | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | 294 | Stephen Ming-teh Lu, PhD Thesis, Purdue University, 2000 (See SKEMPI for data source) | 5.26E-12 | -15.17 | ||||
| 37 | 4CPA | A:I | Bovine carboxypeptidase A1 (CPA) | Solanum tuberosum metallocarboxypeptidase inhibitor (CPI) | EI | Continuous photometric assay with chromogenic substrate | 50mM Tris-HCl, 100mM NaCl | 298 | 7.5 | 10691989 (Table 1, p. 1505) | 1.50E-09 | -12.03 | |
| 38 | 2FTL | E:I | Bovine cationic trypsin/beta-trypsin | Bovine pancreatic trypsin inhibitor (BPTI) | EI | Spectroscopy | 50mM Tris, 20mM CaCl2, 0.01 % (v/v) Triton X-100 | 295 | 8.3 | 10339415 (Table 1, p. 177) | 5.88E-14 | -17.86 | |
| 39 | 4Y0Y | E:I | Bovine cationic trypsin/beta-trypsin | Bovine pancreatic trypsin inhibitor (BPTI) | EI | monitoring the release of p-nitroanilide using spectrophotometry | 50mM Tris-HCl, 20mM CaCl2, 0.005% Triton X-100 | 295 | 8.2 | 8784199 (Table 1, p. 11439) | 5.00E-14 | -17.95 | |
| 40 | 1A4Y | A:B | Bovine ribonuclease A, pancreatic (RNase A) | Human ribonuclease inhibitor, placental (hRI) | EI | measurement of dissociated ligand at various times | 0.1 M Mes, 0.1 M NaCl, 1 mM EDTA | 298 | 6 | 10413501 (Table 2, p. 9278) | 3.10E-14 | -18.42 | |
| 41 | 1A4Y | A:B | Bovine ribonuclease A, pancreatic (RNase A) | Human ribonuclease inhibitor, placental (hRI) | EI | Stopped-flow Fluorescence; Competitive Inhibition | 0.1 M Mes, 0.1 M NaCl, 1mM EDTA, 5mM DTT | 298 | 6 | 10970748 ( Table 2, p. 503, Table 3, p. 504, Tables 4 and 5, p. 508) | 4.30E-14 | -18.23 | |
| 42 | 1A4Y | A:B | Bovine ribonuclease A, pancreatic (RNase A) | Human ribonuclease inhibitor, placental (hRI) | EI | HPLC quantitation of free angiogenin at various times for complexes with native hRI; Scintillation counting of free and bound radiolabeled [14C]Ang for hRI mutants | 0.1 M Mes, containing 0.1 M NaCl, 1 mM EDTA, 120 µM DTT, 100 µg/mL BSA | 298 | 6 | 9050852 (Table 4, p. 1765) | 3.90E-16 | -21.01 | |
| 43 | 2NYY | A:CD | Clostridium botulinum neurotoxin type A | Human monoclonal antibody IgG Fab CR1 | Ag-Ab | Flow cytometry | 296 | 17173035 (Table 2, p. 110) | 1.55E-10 | -13.29 | |||
| 44 | Dendroaspis angusticeps Kunitz-type serine protease inhibitor homolog delta-dendrotoxin | Drosophila melanogaster potassium voltage-gated channel protein shaker | RC | Electrophysiology | 5 mM HEPES, 100mM TEACl, 2 mM KCl, 0.3 mM CaCl2, 1 mM MgCl2 | 298 | 7.6 | 10698633 (Table 1, p. 1287) | 2.00E-09 | -11.86 | |||
| 45 | 1KNE | A:P | Drosophila melanogaster heterochromatin protein 1 (HP1) | Drosophila melanogaster Histone H3 | Fluorescence Anisotropy/Polarization | 50 mM potassium phosphate buffer, 25 mM NaCl, 4 mM DTT | 298 | 8 | 25790265 (Table 1, p. 2316) | 1.70E-05 | -6.5 | ||
| 46 | Enterococcus hirae copper chaperone (CopZ) | Enterococcus hirae probable copper-importing P-type ATPase A (CopA) | OE | Surface Plasmon Resonance | 50mM NaP, 2% acetonitrile, 0.05% dodecylmaltoside | 293 | 7.4 | 11594769 (Table 1, p. 175) | 5.80E-06 | -7.02 | |||
| 47 | Enterococcus hirae copper chaperone (CopZ) | Enterococcus hirae probable copper-importing P-type ATPase A (CopA) | OE | Surface Plasmon Resonance | 50mM NaP, 2% acetonitrile, 0.05% dodecylmaltoside, 10µM copper(I)acetonitrile | 293 | 7.4 | 11594769 (Table 1, p. 175) | 3.60E-07 | -8.64 | |||
| 48 | Enterococcus hirae copper chaperone (CopZ) | Enterococcus hirae probable copper-importing P-type ATPase A (CopA) | OE | Surface Plasmon Resonance | 50mM NaP, 2% acetonitrile, 0.05% dodecylmaltoside, 100µM copper(I)acetonitrile | 293 | 7.4 | 11594769 (Table 1, p. 175) | 7.90E-07 | -8.18 | |||
| 49 | 3VR6 | AD:GH | Enterococcus hirae V-type sodium ATPase catalytic subunits (A3B3) | Enterococcus hirae V-type sodium ATPase catalytic subunits (DG) | OE | Surface Plasmon Resonance | 20mM MES-Tris, 150mM NaCl, 50 µM EDTA-Na, 0.005% Tween 20 | 298 | 6.5 | 24404436 (Table 1, 3rd page) | 1.60E-09 | -11.75 | |
| 50 | 1JTG | A:B | Escherichia coli beta-lactamase (TEM-1) | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | Surface Plasmon Resonance | 10mM Hepes, 3.4mM EDTA, 150mM NaCl, 0.05% surfactant P20 | 298 | 7.4 | 10772866 (Table 1, p. 506) | 6.83E-09 | -11.13 | |
| 51 | 1JTG | A:B | Escherichia coli beta-lactamase (TEM-1) | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | 298 | 10876236 (Table 1, p. 540) | 1.25E-09 | -12.14 | ||||
| 52 | 1JTG | A:B | Escherichia coli beta-lactamase (TEM-1) | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | hydrolysis of cephalosporin C using spectrophotometry | 50mM phosphate buffer with 1 mg/ml BSA | 298 | 7 | 12933802 (Table II, p. 45709) | 5.00E-10 | -12.68 | |
| 53 | 1JTG | A:B | Escherichia coli beta-lactamase (TEM-1) | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | Spectroscopy | 50mM phosphate buffer with 1 mg/ml BSA | 298 | 7 | 15284234 (Table II, p. 42862) | 5.00E-10 | -12.68 | |
| 54 | 1JTG | A:B | Escherichia coli beta-lactamase (TEM-1) | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | Surface Plasmon Resonance | PBS with 0.05% surfactant P20 | 298 | 7.4 | 17070843 (Table 1, p. 669, Table 2, p. 670, Tables 3 and 4, p. 671) | 4.77E-09 | -11.35 | |
| 55 | 1JTG | A:B | Escherichia coli beta-lactamase (TEM-1) | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | Stopped-flow tryptophan fluorescence spectrometry and competitive displacement assay | 10mM Tris, 150mM NaCl | 296 | 7 | 24947275 (Table 3, p. 1239) | 2.30E-10 | -13.05 | |
| 56 | 1JTG | A:B | Escherichia coli beta-lactamase (TEM-1) | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | Spectrophotometric monitoring of cephaloridine hydrolysis | 0.05 M phosphate buffer, 1 mg/ml BSA | 298 | 7 | 9891008 (Table 1, p. 2397) | 1.10E-10 | -13.58 | |
| 57 | 1JTD | A:B | Escherichia coli beta-lactamase (TEM-1) | Streptomyces exfoliatus beta-lactamase inhibitory protein II (BLIP II) | EI | Stopped-flow tryptophan fluorescence spectrometry and competitive displacement assay | 10mM Tris, 150mM NaCl | 296 | 7 | 24947275 (Table 3, p. 1239) | 1.50E-12 | -16.01 | |
| 58 | 1F47 | A:B | Escherichia coli cell division protein/eukaryotic tubulin homolog FtsZ | Escherichia coli cell division protein ZipA | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl, 3mM EDTA, 0.005% (v/v) polysorbate 20 | 298 | 7.5 | 10880432 (Table II, p. 3185) | 2.16E-05 | -6.36 | ||
| 59 | 1F47 | A:B | Escherichia coli cell division protein/eukaryotic tubulin homolog FtsZ | Escherichia coli cell division protein ZipA | ELISA | 10mM Na2HPO4, 1.8mM KH2PO4, 140mM NaCl, 2.7mM KCl, 0.05% Tween 20 | 298 | 7.5 | 11278571 (Table I, p. 11982) | 1.90E-07 | -9.16 | ||
| 60 | 1FFW | A:B | Escherichia coli chemotaxis protein CheA | Escherichia coli chemotaxis protein CheY | OE | Fluorescence-monitored binding titrations | 50mM Tris, 50mM potassium glutamate, 25mM NaCl, 0.5mM DTT, 10% glycerol | 281 | 7 | 21642453 (Table 1, p.3897) | 1.70E-07 | -9.23 | |
| 61 | 1FFG | A:B | Escherichia coli chemotaxis protein CheA | Escherichia coli chemotaxis protein CheY | OE | Isothermal Titration Calorimetry | 20mM sodium phosphate, 20mM NaCl, 1mM EDTA, 1mM PMSF, 10% (v/v) glycerol | 301 | 7.4 | 7578071 (Table 1, p. 14629) | 1.50E-06 | -6.7 | |
| 62 | 1FFW | A:B | Escherichia coli chemotaxis protein CheA | Escherichia coli chemotaxis protein CheY | OE | Fluorescence Quenching | 50 mM sodium phosphate, 5 mM MgCl2 | 298 | 6.5 | 7592655 (Table II, p.24417) | 1.20E-06 | -8.07 | |
| 63 | Escherichia coli colicin-E2 | Escherichia coli colicin-E2 immunity protein (ImmE2) | EI | Subunit exchange method | 50mM MOPS, 200mM NaCl | 298 | 7 | 15019791 (Table 2, p. 748) | 3.90E-15 | -19.65 | |||
| 64 | Escherichia coli colicin-E2 | Escherichia coli colicin-E9 immunity protein (ImmE9) | EI | Subunit exchange method | 50mM MOPS, 200mM NaCl | 298 | 7 | 15019791 (Table 2, p. 748) | 1.20E-08 | -10.8 | |||
| 65 | Escherichia coli colicin-E3 | Escherichia coli colicin-E3 immunity protein (ImmE3) | EI | Isothermal Titration Calorimetry; Fluorescence Anisotropy/Polarization | 50mM MOPS-NaOH, 200mM NaCl | 298 | 7 | 25856265 (Table 1, p. 5253) | 1.20E-12 | -16.25 | |||
| 66 | 2WPT | A:B | Escherichia coli colicin-E9 | Escherichia coli colicin-E2 immunity protein (ImmE2) | EI | Stopped-flow Fluorescence | 50mM Mops buffer, 200mM NaCl, 1mM DTT | 298 | 7 | 18471830 (Table 5, p. 755) | 1.50E-07 | -9.3 | |
| 67 | 2WPT | A:B | Escherichia coli colicin-E9 | Escherichia coli colicin-E2 immunity protein (ImmE2) | EI | Stopped-flow Fluorescence | 50mM Mops buffer, 200mM NaCl, 1mM DTT | 298 | 7 | 9268373 (Table 1, 22256) | 1.47E-08 | -10.68 | |
| 68 | 2WPT | A:B | Escherichia coli colicin-E9 | Escherichia coli colicin-E2 immunity protein (ImmE2) | EI | Stopped-flow Fluorescence | 50mM Mops buffer, 200mM NaCl, 1mM DTT | 298 | 7 | 9718299 (Table 1, p. 11774) | 1.50E-08 | -10.67 | |
| 69 | 1EMV | A:B | Escherichia coli colicin-E9 | Escherichia coli colicin-E9 immunity protein (ImmE9) | EI | Stopped-flow Fluorescence | 50mM Mops buffer, 200mM NaCl, 1mM DTT | 298 | 7 | 18471830 (Table 1, p. 748) | 3.26E-14 | -18.39 | |
| 70 | 1EMV | A:B | Escherichia coli colicin-E9 | Escherichia coli colicin-E9 immunity protein (ImmE9) | EI | Stopped-flow Fluorescence | 50mM Mops buffer, 200mM NaCl, 1mM DTT | 298 | 7 | 9268373 (Table 1, 22256) | 1.82E-14 | -18.74 | |
| 71 | 1EMV | A:B | Escherichia coli colicin-E9 | Escherichia coli colicin-E9 immunity protein (ImmE9) | EI | Stopped-flow Fluorescence | 50mM Mops buffer, 200mM NaCl, 1mM dithiothretol (DTT) | 298 | 7 | 9425068 (Table 1, p. 480) | 1.62E-14 | -18.8 | |
| 72 | 1DKG | AB:D | Escherichia coli protein GrpE/heat shock protein B25.3 | Escherichia coli chaperone protein DnaK/Heat shock protein 70 | Sedimentation Velocity Analytical Ultracentrifugation | 25mM Mopso, 100mM NaCl, 1mM MgCl2, 1mM CaCl2, 5 µM ADP | 293 | 6.8 | 15136046 (Table 1, p. 451) | 2.00E-07 | -8.98 | ||
| 73 | 3BP8 | A:C | Escherichia coli PTS system glucose-specific EIICB component (EIICB-Glc) | Escherichia coli making large colonies protein (mlc) | Surface Plasmon Resonance | 10mM Hepes, 200mM NaCl, 10mM KCl, 1mM MgCl2, 1mM DTT, 0.005% Tween 20 | 298 | 8 | 18319344 (Table 1, p. 3754) | 4.14E-09 | -11.43 | ||
| 74 | 1UUZ | A:D | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Pseudomonas aeruginosa inhibitor of vertebrate lysozyme | EI | Monitoring the change in turbidity due to lysis of cells | 100mM potassium phosphate | 298 | 6.4 | 17405861 (SI Table 3, here) | 1.00E-09 | -12.27 | |
| 75 | 2I26 | N:L | Ginglymostoma cirratum antigen receptor (type II IgNAR) | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Surface Plasmon Resonance | 10mM HEPES, 0.15 M NaCl, 3.4mM EDTA, 0.005% surfactant P20 | 298 | 7.4 | 16446445 (Table 2, p. 1850) | 9.50E-09 | -10.94 | ||
| 76 | 3NGB | A:BC | HIV-1 envelope glycoprotein gp160 subunit gp120 | Human antibody IgG VRC01 | Ag-Ab | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl, 3mM EDTA, and 0.05% surfactant P-20 | 293 | 7.4 | 20616231 (Supporting Online Material, Table S10a, p.41) | 5.76E-09 | -12.33 | |
| 77 | 1GC1 | G:C | HIV-1 envelope glycoprotein gp160 subunit gp120 | Human T-cell surface glycoprotein CD4 | Binding with 125I-labelled recombinant gp120 | 2402498 (Table 1, p.7151) | 3.60E-09 | ||||||
| 78 | 2J0T | A:D | Human 72 kDa type IV collagenase/matrix metalloproteinase-2 (MMP-2) | Human metalloproteinase inhibitor 1/tissue inhibitor of metalloproteinases 1 (TIMP-1) | EI | Fluorescence | 50mM Tris-HCl, 0.15 M NaCl, 10mM CaCl2, and 0.02% Brij 35 | 310 | 7.5 | 12515831 (Table II, p. 9833) | 4.00E-10 | -13.33 | |
| 79 | 1B41 | A:B | Human acetylcholinesterase (AChE) | Dendroaspis angusticeps fasciculin-2 (fas-2) | EI | Enzyme activity assay using the colorimetric detection of AChE product | 50 mM phosphate buffer, 0.1 mg/ml BSA, 0.025% NaN3 (0.15 M) | 298 | 8 | 24613488 (Table 1, p. 639-40) | 9.40E-11 | -13.67 | |
| 80 | 1KAC | A:B | Human adenovirus A serotype 12 fiber protein (ad12 knob) | Human coxsackie virus and adenovirus receptor (CAR) | RC | Fluorescence Anisotropy/Polarization | 10mM Tris-HCl, 0.1mM EDTA, 0.0125% Nonidet P-40 | 298 | 8 | 12716886 (Table 1, p. 26211) | 2.90E-07 | -8.91 | |
| 81 | Human adenovirus A serotype 2 fiber protein (ad2 knob) | Human coxsackie virus and adenovirus receptor (CAR) | RC | Fluorescence Anisotropy/Polarization | 10mM Tris-HCl, 0.1mM EDTA, 0.0125% Nonidet P-40 | 298 | 8 | 12716886 (Table 1, p. 26211) | 3.50E-08 | -10.17 | |||
| 82 | 2J12 | A:B | Human adenovirus D37 fiber protein | Human coxsackie virus and adenovirus receptor (CAR) | RC | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl, 3mM EDTA, 0.005% P20 detergent | 298 | 7.4 | 16923808 (Figure 6, p. 33713) | 2.00E-08 | -10.5 | |
| 83 | 1A4Y | A:B | Human angiogenin | Human ribonuclease inhibitor, placental (hRI) | EI | measurement of dissociated ligand at various times | 0.1 M Mes, 0.1 M NaCl, 1 mM EDTA | 298 | 6 | 10413501 (Table 2, p. 9278) | 3.90E-16 | -21.01 | |
| 84 | 1A4Y | A:B | Human angiogenin | Human ribonuclease inhibitor, placental (hRI) | EI | Stopped-flow Fluorescence; Competitive Inhibition | 0.1 M Mes, 0.1 M NaCl, 1mM EDTA, 120µM DTT | 298 | 6 | 10970748 ( Table 2, p. 503, Table 3, p. 504, Tables 4 and 5, p. 508) | 5.40E-16 | -20.82 | |
| 85 | 1A4Y | A:B | Human angiogenin | Human ribonuclease inhibitor, placental (hRI) | EI | HPLC quantitation of free angiogenin at various times | 0.1 M Mes, 0.1 M NaCl, 1mM EDTA | 298 | 6 | 1281426 (Table VI, p. 12482) | 7.10E-16 | -20.66 | |
| 86 | 1A4Y | A:B | Human angiogenin | Human ribonuclease inhibitor, placental (hRI) | EI | HPLC quantitation of free angiogenin at various times | 0.1 M Mes, containing 0.1 M NaCl, 1 mM EDTA, 120 µM DTT | 298 | 6 | 2479414 (Interaction of H13A- and H114A-Angiogenin with Placental Ribonuclease Inhibitor, p. 7404) | 3.30E-16 | -21.11 | |
| 87 | 1A4Y | A:B | Human angiogenin | Human ribonuclease inhibitor, placental (hRI) | EI | HPLC quantitation of free angiogenin at various times | 0.1 M Mes, 0.1 M NaCl, 1mM EDTA | 298 | 6 | 2742853 (Table I, p.3558) | 7.10E-16 | -20.66 | |
| 88 | 1A4Y | A:B | Human angiogenin | Human ribonuclease inhibitor, placental (hRI) | EI | HPLC quantitation of free angiogenin at various times for complexes with native hRI; Scintillation counting of free and bound radiolabeled [14C]Ang for hRI mutants | 0.1 M Mes, containing 0.1 M NaCl, 1 mM EDTA, 120 µM DTT, 100 µg/mL BSA | 298 | 6 | 9050852 (Table 4, p. 1765) | 3.90E-16 | -21.01 | |
| 89 | 2AJF | A:E | Human angiotensin-converting enzyme 2/ACE-related carboxypeptidase | Human SARS coronavirus spike S glycoprotein | RC | Surface Plasmon Resonance | 0.01 M HEPES, 0.15 M NaCl, 3mM EDTA, 0.005% v/v Surfactant P20 | 298 | 7.4 | 15791205 (Table I, p. 1639) | 1.62E-08 | -10.62 | |
| 90 | 3BDY | V:LH | Human bH1-44 antibody (anti-HER2/anti-VEGF dual-specific antibody) | Human vascular endothelial growth factor A (VEGF A) | Ag-Ab | Surface Plasmon Resonance | PBS with 0.05% Tween 20 | 303 | 21526167 (Fig.2A on 4th page) | 3.30E-09 | -11.76 | ||
| 91 | 3BK3 | A:C | Human bone morphogenetic protein-2 (BMP-2) | Danio rerio crossveinless 2 (cv2) | Surface Plasmon Resonance | 298 | 18477456 (Table 1, p. 745) | 2.20E-08 | -10.44 | ||||
| 92 | 1REW | AB:C | Human bone morphogenetic protein-2 (BMP-2) | Human bone morphogenetic protein receptor type-1A (BMPR-1A) | RC | Surface Plasmon Resonance | 10mM HEPES, 500mM NaCl, 3.4mM EDTA, 0.005% surfactant P20 | 298 | 7.4 | 10880444 (Table 1, p. 3319) | 6.67E-10 | -12.51 | |
| 93 | 1REW | AB:C | Human bone morphogenetic protein-2 (BMP-2) | Human bone morphogenetic protein receptor type-1A (BMPR-1A) | RC | Surface Plasmon Resonance | 10 mM Hepes, 500 mM NaCl, 3.4 mM EDTA, 0.005% Surfactant P20 | 298 | 7.4 | 15064755 (Table 2, p. 484) | 9.00E-10 | -12.33 | |
| 94 | 1REW | AB:C | Human bone morphogenetic protein-2 (BMP-2) | Human bone morphogenetic protein receptor type-1A (BMPR-1A) | RC | Surface Plasmon Resonance | 10mM HEPES, 500mM NaCl, 3.4mM EDTA, 0.005% surfactant P20 | 298 | 7.4 | 18160401 (Table 2, p. 5884) | 7.00E-10 | -13.1 | |
| 95 | Human C-C motif chemokine 11 (eotaxin-1) | Rabbitpox viral CC chemokine inhibitor (35K) | Fluorescence Anisotropy/Polarization | 20 mM potassium phosphate, 100 mM NaCl | 298 | 7 | 24482230 (able 1, p. 6598) | 6.50E-10 | -12.53 | ||||
| 96 | 1GRN | A:B | Human cell division control protein 42 homolog (CDC42) | Human Rho GTPase-activating protein 1/CDC42 GTPase-activating protein (p50-RhoGAP) | GC | monitoring the nucleotide-sensitive fluorescence from Trp97 of Cdc42Hs | 20mM Hepes-NaOH, 5mM MgCl2, 100mM NaCl | 298 | 7.5 | 9632678 (Table 1, p. 16212) | 2.90E-06 | -7.55 | |
| 97 | 2GOX | A:B | Human complement C3 | Staphylococcus aureus fibrinogen-binding protein efb | Surface Plasmon Resonance | 10 mM sodium phosphate, 150 mM sodium chloride, 0.005% Tween-20 | 298 | 8 | 18687868 (Table 2, p. 1899) | 1.40E-09 | -12.07 | ||
| 98 | 2GOX | A:B | Human complement C3 | Staphylococcus aureus fibrinogen-binding protein efb | Isothermal Titration Calorimetry | 20mM Tris, 200mM NaCl | 298 | 8 | 18687868 (Table 2, p. 1899) | 2.00E-09 | -11.86 | ||
| 99 | 2NOJ | A:B | Human complement C3 | Staphylococcus aureus fibrinogen-binding protein efb homolog Ehp | Isothermal Titration Calorimetry | 20mM Tris, 200mM NaCl | 298 | 8 | 17699522 (Table 1, p. 30056) | 1.80E-10 | -13.29 | ||
| 100 | 2HLE | A:B | Human ephrin type-B receptor 4 | Human ephrin-B2 | RC | Isothermal Titration Calorimetry | 50mM Tris-Cl, 150mM NaCl, 1mM CaCl2 | 298 | 7.8 | 16867992 (Table 3, p. 28191) | 4.00E-08 | -10.09 | |
| 101 | Human fibroblast growth factor receptor substrate 2/signaling adaptor SNT-1 | Human high affinity nerve growth factor receptor/neurotrophic tyrosine kinase receptor type 1 (Trk-A) | Isothermal Titration Calorimetry | 50mM Na2PO4, 200mM NaCl | 298 | 7 | 11877385 (Table I, p. 17090) | 7.70E-06 | -6.97 | ||||
| 102 | Human fibroblast growth factor receptor substrate 2/signaling adaptor SNT-1 | Human high affinity nerve growth factor receptor/neurotrophic tyrosine kinase receptor type 1 (Trk-A) | Isothermal Titration Calorimetry | 20mM Tris-HCl, 200mM NaCl | 298 | 8 | 11877385 (Table I, p. 17090) | 1.53E-05 | -6.57 | ||||
| 103 | 1GCQ | AB:C | Human growth factor receptor-bound protein 2 (GRB2) | Mouse proto-oncogene Vav1, SH3 domain | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl, 3mM EDTA, 0.005% (v/v) polysorbate 20, 2.5mM 2-mercaptoethanol | 298 | 7.4 | 11406576 (Table III, p. 3003) | 1.68E-05 | -6.51 | ||
| 104 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 1 (mAb 1) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -13.1 | ||||
| 105 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 10 (mAb 10) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -10.6 | ||||
| 106 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 11 (mAb 11) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -12.2 | ||||
| 107 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 12 (mAb 12) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -13.1 | ||||
| 108 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 13 (mAb 13) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -12.6 | ||||
| 109 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 14 (mAb 14) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -13.5 | ||||
| 110 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 15 (mAb 15) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -12.7 | ||||
| 111 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 16 (mAb 16) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -13.1 | ||||
| 112 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 17 (mAb 17) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -12.8 | ||||
| 113 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 18 (mAb 18) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -12.85 | ||||
| 114 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 19 (mAb 19) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -13.1 | ||||
| 115 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 2 (mAb 2) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -13.1 | ||||
| 116 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 20 (mAb 20) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -13.1 | ||||
| 117 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 21 (mAb 21) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -12.45 | ||||
| 118 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 3 (mAb 3) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -13.1 | ||||
| 119 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 4 (mAb 4) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -11.2 | ||||
| 120 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 5 (mAb 5) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -14.2 | ||||
| 121 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 6 (mAb 6) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -10.8 | ||||
| 122 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 7 (mAb 7) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -13.1 | ||||
| 123 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 8 (mAb 8) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -10.1 | ||||
| 124 | Human growth hormone/somatotropin (hGH) | anti-hGH mouse monoclonal antibody 9 (mAb 9) | Ag-Ab | Robot-aided enzyme-linked immunoabsorbant assay | 50 mM Tris, 0.15 M NaCl, 2 mM EDTA, 5 mg/mL BSA (in 0.05% (w/v) Tween 20 and 0.02% (w/v) sodium azide) | 298 | 7.5 | 1380563 (Table 4, p. 855-6) | -10.1 | ||||
| 125 | 1A22 | A:B | Human growth hormone/somatotropin (hGH) | Human growth hormone receptor (hGHR) | HR | Competitive displacement of 125I-labeled hGH | 50 mM Tris-HCl, 10 mM CaCl2, 0.1% BSA, 0.02% NaN3 | 298 | 7.4 | 2014261 (Table 2, p. 3409) | 4.60E-10 | -12.73 | |
| 126 | 1A22 | A:B | Human growth hormone/somatotropin (hGH) | Human growth hormone receptor (hGHR) | HR | Competitive displacement of 125I-labeled hGH | 50 mM Tris-HCl, 10 mM CaCl2, 0.1% BSA, 0.02% NaN3, 50 µM ZnCl2, 10 mM MgCl2 | 298 | 7.4 | 2014261 (Table 4, p. 3409) | 4.40E-10 | -12.76 | |
| 127 | 1A22 | A:B | Human growth hormone/somatotropin (hGH) | Human growth hormone receptor (hGHR) | HR | Competitive displacement of 125I-labeled hGH | 50 mM Tris-HCl, 10 mM CaCl2, 1 g/L BSA, 0.2 g/L NaN3 | 298 | 7.4 | 2034689 (Table 1, p. 4499) | 4.00E-10 | -12.81 | |
| 128 | 1A22 | A:B | Human growth hormone/somatotropin (hGH) | Human growth hormone receptor (hGHR) | HR | Competitive displacement of 125I-labeled hGH | 50 mM Tris-HCl, 10 mM CaCl2, 1 g/L BSA, 0.2 g/L NaN3 | 298 | 7.4 | 2471267 (Table 1, p. 1082) | 3.40E-10 | -12.91 | |
| 129 | 1A22 | A:B | Human growth hormone/somatotropin (hGH) | Human growth hormone receptor (hGHR) | HR | Surface Plasmon Resonance | 150 mM NaCl, 10 mM sodium phosphate, 0.02% Tween 20 | 298 | 7.4 | 7504735 (Tables 1 and 2, pp. 556-7) | 9.00E-10 | -12.33 | |
| 130 | 1A22 | A:B | Human growth hormone/somatotropin (hGH) | Human growth hormone receptor (hGHR) | HR | Competitive Binding | 50mM Tris-HCl, 10mM CaCl2, 1g/L bovine albumin, 0.2 g/L NaN3 | 298 | 7.4 | 7529940 (Figure 2, p. 384) | 9.63E-10 | -12.29 | |
| 131 | 1A22 | A:B | Human growth hormone/somatotropin (hGH) | Human growth hormone receptor (hGHR) | HR | Surface Plasmon Resonance | PBS with 10mM sodium phosphate, 137mM NaCl, 2.7mM KCl, 0.05% Tween 20 | 298 | 7.2 | 8756685 (Table 2, p. 10303) | 2.90E-09 | -11.64 | |
| 132 | 1A22 | A:B | Human growth hormone/somatotropin (hGH) | Human growth hormone receptor (hGHR) | HR | competitive displacement of hGH from hGHbp, using 125I-labeled hGH as a tracer | PBS with 0.02% (v/v) Tween 20 | 298 | 9571026 (Table 1, p. 1118) | 3.40E-10 | -12.91 | ||
| 133 | 1BP3 | A:B | Human growth hormone/somatotropin (hGH) | Human prolactin receptor (hPRL-R) | HR | Competitive displacement of 125I-labeled hGH | 50 mM Tris-HCl, 10 mM CaCl2, 0.1% BSA, 0.02% NaN3, 50 µM ZnCl2, 10 mM MgCl2 | 298 | 7.4 | 2014261 (Table 2, p. 3409) | 3.30E-11 | -14.29 | |
| 134 | 1BP3 | A:B | Human growth hormone/somatotropin (hGH) | Human prolactin receptor (hPRL-R) | HR | Competitive displacement of 125I-labeled hGH | 50 mM Tris-HCl, 10 mM CaCl2, 0.1% BSA, 0.02% NaN3, 1 mM EDTA | 298 | 7.4 | 2014261 (Table 3, p. 3409) | 2.70E-07 | -8.96 | |
| 135 | 1FYT | AB:C | Human HLA class II histocompatibility antigen DR (MHCII HLA-DR) | Influenza A virus hemagglutinin | Competitive Peptide Binding Assay | 0.1% BSA, 0.01% Tween 20, 0.1 mg/mL 4-(2-aminoethyl)-benzene sulfonyl fluoride 0.1mM iodoacetamide, 5mM EDTA, 0.02% NaN3 | 310 | 7.2 | 15823020 (Table 1, p. 5618) | 8.53E-08 | -10.03 | ||
| 136 | 1FCC | A:C | Human Ig gamma-1 chain C region (IgG1 MO61) Fc | Streptococcus sp. group G immunoglobulin G-binding protein G | Ag-Ab | Fluorescence | 20mM phosphate | 298 | 6 | 10452608 (Table 1, p. 1644) | 2.40E-07 | -9.03 | |
| 137 | 4OGA | AB:E | Human insulin receptor, soluble (sHIR) | Human insulin | HR | Microtiter Plate Antibody Capture Assay | 277 | 7852381 (Table I, p.3014) | 1.41E-09 | -11.22 | |||
| 138 | 4OGA | AB:E | Human insulin receptor, soluble (sHIR) | Human insulin | HR | Microtiter Plate Antibody Capture Assay | 137.5mM NaCl, 10mM magnesium sulfate, 20 mM Hepes, 0.5% BSA (w/v), 0.025% Triton X-100 (v/v), and 0.02% sodium azide (w/v) | 277 | 7.8 | 8576204 (Table I, p. 2441) | 5.60E-10 | -11.73 | |
| 139 | 4XSS | B:E | Human insulin-like growth factor I (IGF-I) | Human insulin receptor (IR) | HR | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl, 3.4mM EDTA, 0.05% P20 | 298 | 7.4 | 9733769 (Table II, p. 24704) | 5.78E-09 | -11.23 | |
| 140 | 1WQJ | I:B | Human insulin-like growth factor I (IGF-I) | Human insulin-like growth factor-binding protein-1 (IGFBP-1) | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl, 3.4mM EDTA, 0.05% P20 | 298 | 7.4 | 9733769 (Table II, p. 24704) | 4.14E-10 | -12.79 | ||
| 141 | 1MQ8 | A:B | Human integrin αLβ2 | Human intercellular adhesion molecule 1 (ICAM-1) | Surface Plasmon Resonance | Tris buffer with 1mM MgCl2 | 298 | 12526797 (Table 1, p. 100) | 1.50E-03 | -3.85 | |||
| 142 | 1MQ8 | A:B | Human intercellular adhesion molecule 1 (ICAM-1) | Human integrin alpha-L (LFA-1A) | Surface Plasmon Resonance | Tris-buffered saline with 1mM MgCl2 | 298 | 12526797 (Table 1, p. 100) | 3.00E-06 | -7.53 | |||
| 143 | Human interleukin-18 (hIL-18) | Human interleukin-18 binding protein (hIL-18BP) | Surface Plasmon Resonance | 0.01 M Hepes, 0.15 M NaCl, 3mM EDTA, 0.1% Tween 20 | 298 | 7.4 | 11278524 (Table 1, p. 17385) | 6.00E-11 | -13.94 | ||||
| 144 | 2B5I | A:BD | Human interleukin-2 (hIL-2) | Human interleukin-2 receptor alpha-beta complex (hIL-2R/p55-p70) | RC | Direct binding with radiolabeled Na125I | 2052547 (Table 2, p. 4638) | 5.40E-11 | |||||
| 145 | 2B5I | A:B | Human interleukin-2 (hIL-2) | Human interleukin-2 receptor beta (hIL-2Rb) | RC | Direct binding with radiolabeled Na125I | 2052547 (Table 2, p. 4638) | 3.60E-10 | |||||
| 146 | 1IAR | A:B | Human interleukin-4 (hIL-4) | Human interleukin-4 receptor alpha (hIL-4Ra) | RC | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl | 298 | 7.4 | 11786020 (Table 1, p. 400) | 1.50E-10 | -13.40 | |
| 147 | 1IAR | A:B | Human interleukin-4 (hIL-4) | Human interleukin-4 receptor alpha (hIL-4Ra) | RC | Surface Plasmon Resonance | 10mM Hepes, 150mM NaCl, 3.4mM EDTA, 0.005% surfactant P20 | 298 | 7.4 | 9050834 (Table 1, p. 1658) | 1.62E-10 | -13.35 | |
| 148 | 3BPL | AB:C | Human interleukin-4 (IL-4) with interleukin-4 receptor (IL-4R) | Cytokine receptor common subunit gamma | RC | Surface Plasmon Resonance | 10mM Hepes, 150mM NaCl, 3.4mM EDTA, 0.005% surfactant P20 | 298 | 7.4 | 9799097 (Table 1, p. 15) | 3.44E-06 | -7.45 | |
| 149 | 1ILQ | AB:C | Human interleukin-8 (hIL-8) | Human interleukin-8 receptor (hIL-8R) | RC | Binding measured using 125I-IL-8 | 25mM Hepes, 0.5% BSA, HBSS without Mg2+ or Ca2+ | 277 | 7.2 | 8034699 (Table I, p. 19344) | 3.03E-09 | -10.8 | |
| 150 | 1ILQ | AB:C | Human interleukin-8 (hIL-8) | Human interleukin-8 receptor (hIL-8R) | RC | Binding measured using 125I-IL-8 | 277 | 8103045 (Table 2, p. 18551) | 9.70E-10 | -11.42 | |||
| 151 | Human leptin | Human leptin receptor | HR | Surface Plasmon Resonance | 0.01 M HEPES, 0.15 M NaCl, 3mM EDTA, 0.005% v/v Surfactant P20 | 298 | 7.4 | 24743494 (Table 1, p. 4) | 6.47E-11 | -13.89 | |||
| 152 | 1EAW | A:B | Human membrane-type serine protease 1/suppressor of tumorigenicity 14 protein (matriptase/MT-SP1) | Bovine pancreatic trypsin inhibitor (BPTI) | EI | Fluorescence | 50mM Tris-HCl, 50mM NaCl, 0.01% (v/v) Tween-20 | 298 | 8.8 | 17475279 (Table 2, p. 1046) | 4.97E-11 | -14.05 | |
| 153 | 3BN9 | B:CD | Human membrane-type serine protease 1/suppressor of tumorigenicity 14 protein (matriptase/MT-SP1) | Human Ig heavy chain V-III region 23 (E2 Fab) | Ag-Ab | Stopped-flow Spectroscopy | 298 | 17475279 (Table 2, p. 1046) | 1.23E-11 | -14.88 | |||
| 154 | 3NPS | A:BC | Human membrane-type serine protease 1/suppressor of tumorigenicity 14 protein (matriptase/MT-SP1) | Human S4 Fab | Ag-Ab | Stopped-flow Spectroscopy | 298 | 17475279 (Table 2, p. 1046) | 7.04E-11 | -13.84 | |||
| 155 | 2VLJ | AC:DE | Human MHCI-A2/Infuenza A virus matrix peptide complex | Human T-cell receptor JM22 Vβ17 | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl, 3.4mM EDTA, and 0.005% Surfactant P20 | 298 | 7.4 | 18275829 (Table 2, p. 176, Table 3, p. 177) | 5.20E-06 | -7.21 | ||
| 156 | 1PPF | E:I | Human neutrophil elastase (elastase-2/HLE) | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Spectroscopy | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 11171964 (data previously accessible online here, please check SKEMPI Notes for details on the data source) | 1.64E-10 | -13.16 | |
| 157 | 1PPF | E:I | Human neutrophil elastase (elastase-2/HLE) | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Spectroscopy | 0.1 M Tris-HCl, 0.025% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 12767228 (Tables 1 and 2, p. 6462, Table 3, p. 6463) | 1.53E-10 | -13.2 | |
| 158 | 1PPF | E:I | Human neutrophil elastase (elastase-2/HLE) | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Titration | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 9047374 (Table 1, p. 443) | 1.64E-10 | -13.16 | |
| 159 | 1PPF | E:I | Human neutrophil elastase (elastase-2/HLE) | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | 294 | Stephen Ming-teh Lu, PhD Thesis, Purdue University, 2000 (See SKEMPI for data source) | 1.64E-10 | -13.16 | ||||
| 160 | 2J0T | A:D | Human nterstitial collagenase/matrix metalloproteinase-1 (MMP-1) | Human metalloproteinase inhibitor 1/tissue inhibitor of metalloproteinases 1 (TIMP-1) | EI | activity measured by following release of fluorogenic product with time | 50mM Tris-HCl, 0.15 M NaCl, 10mM CaCl2, 0.02% Brij 35 | 310 | 7.5 | 9268350 (Table II, p. 22089) | 1.50E-09 | -12.52 | |
| 161 | 1AK4 | A:D | Human peptidyl-prolyl cis-trans isomerase A/cyclophilin A (CypA) | HIV-1 gag-pol polyprotein/capsid protein (CA) | Surface Plasmon Resonance | 100mM NaCl, 25mM potassium phosphate, 1mM β-mercaptoethanol, 0.005% (v/v) P20 | 293 | 6.5 | 9223641 ( Table 2, p. 787) | 1.20E-06 | -7.94 | ||
| 162 | Human peptidyl-prolyl cis-trans isomerase A/cyclophilin A (hCypA) | Human SARS coronavirus nucleocapsid protein (NP) | Surface Plasmon Resonance | 10mM Hepes, 150mM NaCl, 3.4mM EDTA, 0.005% (v/v) surfactant P20 | 298 | 7.4 | 15358143 (Table 1, p. 561) | 6.04E-09 | -11.21 | ||||
| 163 | 2C0L | A:B | Human peroxisomal targeting signal 1 receptor/peroxin-5 (PEX5) TPR domain | Human non-specific lipid-transfer protein/sterol carrier protein 2 (NSL-TP/SCP-2) | Isothermal Titration Microcalorimetry | 100mM potassium phosphate, 1mM DTT | 308 | 7.4 | 17157249 (Table 1, p. 655) | 1.09E-07 | -9.81 | ||
| 164 | 1HE8 | A:B | Human phosphatidylinositol 4,5-bisphosphate 3-kinase (PI3K) | Human GTPase HRas | OE | Fluorescence Anisotropy/Polarization | 20mM Tris, 2mM MgCl2, 1mM DTT | 293 | 7.5 | 11136978 (Figure 2, p. 933) | 1.30E-06 | -7.89 | |
| 165 | 1DAN | TU:LH | Human plasma coagulation factor VIIa | Human tissue factor/thromboplastin | OE | Surface Plasmon Resonance | 20mM Tris-HCl, 100mM NaCl, 5mM CaC12 | 298 | 7.5 | 7654692 (Figure 4, pp. 10387) | 6.16E-09 | -11.2 | |
| 166 | 1DAN | TU:LH | Human plasma coagulation factor VIIa | Human tissue factor/thromboplastin | OE | Enzyme-Linked Functional Assay | 20mM HEPES, 130mM NaCl, 5mM CaC12, 0.5% BSA | 310 | 7.4 | 7756258 (Table 2, p.6312) | 2.60E-12 | -16.43 | |
| 167 | 1DAN | TU:LH | Human plasma coagulation factor VIIa | Human tissue factor/thromboplastin | OE | ELISA | 277 | 7947809 (Table 3, p. 14008) | 5.30E-09 | -10.49 | |||
| 168 | 1DAN | TU:LH | Human plasma coagulation factor VIIa | Human tissue factor/thromboplastin | OE | Linked Chromogenic Assay | 5mM Ca2+ | 310 | 8312277 (Figure 3, p.1569) | 4.10E-12 | -16.15 | ||
| 169 | 1DAN | TU:LH | Human plasma coagulation factor VIIa | Human tissue factor/thromboplastin | OE | Surface Plasmon Resonance | 10mM Hepes, 150mM NaCl, 5mM CaCl2, 1mM CHAPS, 0.005% surfactant P20 | 295 | 7.4 | 8962059 (Table 1, p. 14382) | 3.70E-09 | -11.38 | |
| 170 | Human prohibitin-2 (PHB2/REA) | Human estrogen receptor alpha (ER-α) | RC | Isothermal Titration Calorimetry | 20 mM HEPES-NaOH, 150 mM NaCl, 5% glycerol, 0.5 mM TCEP | 298 | 8 | 26049107 (Table 1, p. 729) | 1.30E-06 | -8.03 | |||
| 171 | Human protein-glutamine gamma-glutamyltransferase 2/transglutaminase-2 (TG2) | Human fibronectin (FN) | Surface Plasmon Resonance | 0.01 M Hepes, 0.15 M NaCl, 3 mM EDTA, 0.005 % surfactant P20 | 298 | 7.4 | 27394141 (Table I on the 8th page) | 9.00E-10 | -12.33 | ||||
| 172 | 1GUA | A:B | Human RAF proto-oncogene serine/threonine-protein kinase (c-Raf) | Human Ras-related protein Rap-1A | Isothermal Titration Calorimetry | 50mM Tris-HCl, 5mM MgCl2, 100mM NaCl | 298 | 7.4 | 15236966 (Tables 3, 4 and 6, pp. 1046, 1047 and 1051 respectively) | 4.90E-07 | -8.6 | ||
| 173 | 1LFD | AC:BD | Human Ral guanine nucleotide dissociation stimulator (RalGDS) | Human GTPase HRas | Isothermal Titration Calorimetry | 50mM Tris-HCl, 5 mM MgCl2 | 298 | 7.4 | 15236966 (Tables 3, 4 and 6, pp. 1046, 1047 and 1051 respectively) | 6.90E-07 | -8.4 | ||
| 174 | 1LFD | AC:BD | Human Ral guanine nucleotide dissociation stimulator (RalGDS) | Human GTPase HRas | Fluorescence | 50mM Tris-HCl, 5 mM MgCl2 | 310 | 7.4 | 15236966 (Tables 3, 4 and 6, pp. 1046, 1047 and 1051 respectively) | 1.20E-06 | -8.4 | ||
| 175 | Human Ral guanine nucleotide dissociation stimulator (RalGDS) | Human Ras-related protein Rap-1A | Isothermal Titration Calorimetry | 50mM Tris-HCl, 5 mM MgCl2 | 298 | 7.4 | 15236966 (Tables 3, 4 and 6, pp. 1046, 1047 and 1051 respectively) | 5.50E-08 | -9.9 | ||||
| 176 | 1E96 | A:B | Human ras-related C3 botulinum toxin substrate 1 (Rac-1) | Human neutrophil cytosol factor 2 (p67phox, NCF2) | Fluorescence spectrophotometric assay | 20mM Tris acetate buffer with 3mM NaCl, 50mM KCl, 0.1 µm MgCl2 | 293 | 7.45 | 9228059 (Table 1, p. 18837) | 1.24E-07 | -9.26 | ||
| 177 | 2A9K | A:B | Human ras-related protein Ral-A | Clostridium botulinum D phage mono-ADP-ribosyltransferase C3/exoenzyme C3 | Isothermal Titration Calorimetry | 20mM Tris-HCl, 50mM NaCl, 2mM MgCl2, 2mM DTE | 310 | 7.5 | 16177825 (Table 1, p. 3671) | 6.00E-08 | -10.24 | ||
| 178 | Human ras-related protein Ral-B | Human exocyst complex component 2 (Sec5) | GC | Scintillation Proximity Assay | 20 mM Tris-HCl, 2 mM MgCl2, 1 mM DTT, 0.2 mg/mL BSA | 7.5 | 25621740 (Table 3, p. 1385) | 2.38E-07 | |||||
| 179 | 2KWI | A:B | Human ras-related protein Ral-B | Human ralA-binding protein 1 (RalBP1) | GC | Scintillation Proximity Assay | 20 mM Tris-HCl, 2 mM MgCl2, 1 mM DTT, 0.2 mg/mL BSA | 7.5 | 25621740 (Table 3, p. 1385) | 2.16E-07 | |||
| 180 | 4OZ1 | AB:C | Human RNA-binding protein 39 (CAPER-α) | Human splicing factor 3B subunit 1 (SF3b155) | Isothermal Titration Calorimetry | 50 mM NaCl, 25 mM HEPES, 0.2 mM TCEP | 303 | 7.4 | 24795046 (Table 2, p.17330) | 5.80E-08 | -10.03 | ||
| 181 | 1E50 | A:B | Human runt-related transcription factor 1/core-binding factor alpha (CBF-α) | Human core binding factor beta (CBF-β) | Electrophoretic Mobility Shift Assay | 10984496 (Table III, p. 39584) | 4.50E-08 | 0 | |||||
| 182 | 1E+50 | A:B | Human runt-related transcription factor 1/core-binding factor alpha (CBF-α) | Human core binding factor beta (CBF-β) | Electrophoretic Mobility Shift Assay | 273 | 12807883 (Table III and IV, p. 33101) | 1.40E-08 | -9.81 | ||||
| 183 | 1UEA | A:B | Human stromelysin-1/matrix metalloproteinase-3 (MMP-3) | Human metalloproteinase inhibitor 1/tissue inhibitor of metalloproteinases 1 (TIMP-1) | EI | Fluorescence | 50mM Tris-HCl, 0.15 M NaCl, 10mM CaCl2, and 0.02% Brij 35 | 310 | 7.5 | 12515831 (Table II, p. 9833) | 2.00E-10 | -13.76 | |
| 184 | 1AO7 | AC:DE | Human T-cell receptor A6 | Human HLA class I histocompatibility antigen, A-2 alpha chain with protein TAX-1 (TAX-HLAA2) | Ag-Ab | Surface Plasmon Resonance | 0.01 M HEPES, 0.15mM NaCl, 3mM EDTA, and 0.005% (v/v) Surfactant P20 | 298 | 7.4 | 20681514 (Table 1, p. 7052) | 2.10E-06 | -7.74 | |
| 185 | 1AO7 | AC:DE | Human T-cell receptor A6 | Human HLA class I histocompatibility antigen, A-2 alpha chain with protein TAX-1 (TAX-HLAA2) | Ag-Ab | Surface Plasmon Resonance | 0.01 M Hepes, 0.15mM NaCl, 3mM EDTA and 0.005% (v/v) Surfactant P20 | 298 | 7.4 | 24380763 (Table 2, p.1591) | 2.20E-06 | -7.71 | |
| 186 | Human tetranectin (TN) | Human plasminogen (kringle 4 protein domain) | Surface Plasmon Resonance | 50mM Tris-HCl, 0.1 M NaCl, 0.005% Surfactant P20 | 298 | 8 | 9786936 (Table II, p. 29244) | 5.50E-07 | -8.54 | ||||
| 187 | Human tetranectin (TN) | Human plasminogen (kringle 4 protein domain) | Surface Plasmon Resonance | 50mM Tris-HCl, 500mM NaCl | 298 | 8 | 9786936 (Table II, p. 29244) | 5.50E-07 | -8.54 | ||||
| 188 | 1KTZ | A:B | Human transforming growth factor beta 3 (TGF-β3) | Human TGF-beta receptor type-2 (TGFR-2) | RC | Surface Plasmon Resonance | 20mM Hepes, 150mM NaCl, 3.4mM EDTA, 0.005% Tween-20 | 298 | 7.4 | 16300789 (Table 1, p. 53) | 3.20E-07 | -8.86 | |
| 189 | 1KTZ | A:B | Human transforming growth factor beta 3 (TGF-β3) | Human TGF-beta receptor type-2 (TGFR-2) | RC | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl, 3.4mM EDTA, 0.005% Tween 20 | 298 | 7.4 | 19161338 (Table 1, p. 2150) | 7.30E-08 | -9.73 | |
| 190 | 1S1Q | A:B | Human tumor susceptibility gene 101 protein (Tsg101) | Human polyubiquitin-C | Surface Plasmon Resonance | 20mM sodium phosphate, 150mM NaCl, 0.05% BSA, 0.01% P20 | 293 | 7.2 | 12006492 (Table II, p.2401) | 6.35E-04 | -4.29 | ||
| 191 | 1XD3 | A:B | Human ubiquitin carboxyl-terminal hydrolase isozyme L3 (UCH-L3) | Human polyubiquitin-C | Competitive Inhibition | 50mM Tris-HCl, 10mM DTT | 310 | 7.5 | 10518943 (Table 1, p. 1071) | 3.00E-07 | -9.25 | ||
| 192 | 2OOB | A:B | Human ubiquitin protein ligase Cbl-b | Human polyubiquitin-C | Fluorescence titration | 298 | 18596201 (Table 2, p. 1813) | 5.31E-05 | -5.83 | ||||
| 193 | 1C4Z | A:D | Human ubiquitin-conjugating enzyme E2 L3 (UbcH7) | Human ubiquitin-protein ligase E3A (E6AP) | OE | Fluorescence Anisotropy/Polarization | 20mM KH2PO4, 150mM NaCl, 5mM β-ME | 298 | 7 | 17433363 (Table 1, p. 422) | 5.00E-06 | -7.23 | |
| 194 | Human UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) | Human alpha-actinin 1 | Surface Plasmon Resonance | 20mM Tris, 100-300mM NaCl, 0.1mM DTT, 2mM CaCl2 | 298 | 7.5 | 18560563 (Table 2, 5th page) | 6.80E-08 | -13.05 | ||||
| 195 | Human UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE) | Human alpha-actinin 2 | Surface Plasmon Resonance | 20mM Tri, 250mM NaCl, 0.1mM DTT, 2 mM CaCl2 | 398 | 7.5 | 27023225 (Figure 2 on the 5th page) | 9.37E-09 | -14.62 | ||||
| 196 | 2I9B | A:E | Human urokinase plasminogen activator receptor (uPAR) | Human single chain urokinase-type plasminogen activator (scuPA) | RC | Surface Plasmon Resonance | PBS with 0.005% Tween 20 | 298 | 7.4 | 10864923 (Table III, p. 28536) | 9.10E-10 | -12.33 | |
| 197 | 2I9B | A:E | Human urokinase plasminogen activator receptor (uPAR) | Human single chain urokinase-type plasminogen activator (scuPA) | RC | Surface Plasmon Resonance | PBS with 0.005% Tween 20 | 298 | 7.4 | 10864923 (Tables II and III, p. 28535-6) | 3.30E-10 | -12.93 | |
| 198 | Human vascular endothelial growth factor A (VEGF A) | Human vascular endothelial growth factor receptor 2 (VEGFR-2)/kinase insert domain receptor (KDR) | Ag-Ab | Radioimmunoassay | PBS, 0.5% BSA, 0.1% Tween 20 | 9207067 (Tables 2 and 3, p. 7195) | -14.7 | ||||||
| 199 | 1BJ1 | W:LH | Human vascular endothelial growth factor A (VEGF A) | Humanized mouse antibody mAb A4.6.1 | Ag-Ab | Radioimmunoassay | PBS, 0.5% BSA, 0.1% Tween 20 | 9207067 (Tables 2 and 3, p. 7195) | 3.41E-09 | -16.01 | |||
| 200 | 1CZ8 | V:LH | Human vascular endothelial growth factor A (VEGF A) | Mouse antibody Y0192/humanized anti-VEGF Fab-12 | Ag-Ab | Surface Plasmon Resonance | PBS with 0.05% Tween 20 | 298 | 10543973 (Table 6, p. 870) | 2.90E-09 | -11.64 | ||
| 201 | 3F1S | A:B | Human vitamin-K dependent protein Z | Human protein Z-dependent protease inhibitor (ZPI/serpin A10) | EI | Competitive binding titrations | Tris buffer, 0.1 M NaCl | 298 | 7.1 | 25713144 (Table 2, p. 9911) | 2.10E-09 | -11.83 | |
| 202 | Humanized mouse monoclonal antibody Fab (mAb hu4D5-5) | Human epidermal growth factor receptor 2 (EGFR-2/HER2) | Ag-Ab | Surface Plasmon Resonance | 20mM sodium phosphate, 0.1 M NaCl, 0.05% Tween 20 | 298 | 7.5 | 7687461 (Table 1, p.6831) | 2.70E-10 | -13.05 | |||
| 203 | 1YY9 | A:CD | Human-mouse monoclonal antibody C225 gamma 1 Fab (cetuximab) | Human epidermal growth factor receptor 2 (EGFR-2/HER2) | Ag-Ab | Surface Plasmon Resonance | 0.01 M Hepes, 0.15mM NaCl, 3mM EDTA and 0.005% (v/v) Surfactant P20 | 298 | 7.4 | 24380763 (Table 4, p. 1593) | 1.31E-09 | -12.11 | |
| 204 | 1NMB | N:LH | Influenza A virus neuraminidase subtype N9 | Mouse antibody NC10 Fab | Ag-Ab | Surface Plasmon Resonance | 10mM HEPES, 0.15 M NaCl, 3.4mM EDTA, 0.005% surfactant P20 | 298 | 7.4 | 9579662 (Table IV, p. 71) | 4.55E-08 | -10.01 | |
| 205 | 1NCA | N:LH | Influenza A virus neuraminidase subtype N9 | Mouse Ig gamma-2A chain C region/antibody NC41 scFv | Ag-Ab | Fluorescence Quenching Assay | PBS | 298 | 7.2 | 9692956 (Table 2, p. 10667) | 5.00E-08 | -9.96 | |
| 206 | 3N4I | A:B | Klebsiella pneumoniae beta-lactamase SHV-1 | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | Spectroscopy | 50 mm phosphate buffer with 1 mg/ml BSA | 298 | 7 | 15284234 (Table II, p. 42862) | 1.13E-06 | -8.11 | |
| 207 | 2G2U | A:B | Klebsiella pneumoniae beta-lactamase SHV-1 | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | Nitrocefin indicator to measure hydrolysis | 10mM sodium PBS with 1 mg/ml BSA | 298 | 7.4 | 16809340 (Table 1, p. 26746) | 1.25E-06 | -8.05 | |
| 208 | 3C4P | A:B | Klebsiella pneumoniae beta-lactamase SHV-1 | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | sodium PBS with 100 g/ml BSA | 18775544 (Table 3, p. 1269) | 1.72E-06 | |||||
| 209 | 1SBB | A:B | Mouse 14.3.D T-cell antigen receptor | Staphylococcus aureus enterotoxin type B (SEB) | Sedimentation equilibrium using analytical centrifugation or surface plasmon resonance | 50 mM Tris-HCl | 298 | 7.5 | 9500785 (Table 1, p. 826) | 1.40E-04 | -5.25 | ||
| 210 | 1JCK | AC:BD | Mouse 14.3.D T-cell antigen receptor | Staphylococcus aureus enterotoxin type C-3 (SEC3) | Surface Plasmon Resonance | 150mM NaCl, 10mM Na-Hepes, 3.4mM EDTA, 0.005% Surfactant P20 | 298 | 7.5 | 10704464 (Table 1, p. 840) | 1.85E-07 | -9.18 | ||
| 211 | 2DVW | A:B | Mouse 26S proteasome non-ATPase regulatory subunit 10 (gankyrin) | Human 26S protease regulatory subunit 6B | OE | Isothermal Titration Calorimetry | 20 mM sodium phosphate, 150 mM NaCl, 2.5 mM 2-mercaptoethanol | 298 | 7.4 | 25343477 (Table 1, p. 6858) | 6.73E-08 | -9.78 | |
| 212 | 1KU6 | A:B | Mouse acetylcholinesterase (AChE) | Dendroaspis angusticeps fasciculin-2 (fas-2) | EI | Measure of substrate hydrolysis after attaining equilibrium | 0.1 M NaPO4 buffer, with 0.01% BSA | 310 | 7 | 8157652 (Table 1, p. 11235) | 2.30E-12 | -16.51 | |
| 213 | 2BDN | A:LH | Mouse antibody 11K2 scFv | Human C-C motif chemokine 2/monocyte chemoattractant protein 1 (MCP-1) | Ag-Ab | Surface Plasmon Resonance | PBS 0.005% (v/v) Tween20 | 298 | 24475232 (Table 1 on 4th page) | 8.00E-10 | -12.4 | ||
| 214 | 1VFB | AB:C | Mouse anti-hen egg white lysozyme monoclonal antibody D1.3 | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | Surface Plasmon Resonance | 150mM NaCl, 0.005% Surfactant P-20, 10mM Hepes | 298 | 7.5 | 8703938 (Table 1, p. 9672) | 2.00E-08 | -10.5 | |
| 215 | 1KIR | AB:C | Mouse anti-hen egg white lysozyme monoclonal antibody D1.3 | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | Isothermal Titration Calorimetry | 0.15 M NaCl, 0.01 M Tris-HCl | 298 | 7.2 | 8952503 (Table 3, p. 15498) | 3.85E-08 | -10.11 | |
| 216 | 1VFB | AB:C | Mouse anti-hen egg white lysozyme monoclonal antibody D1.3 | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | Surface Plasmon Resonance | 150mM NaCl, 0.005% Surfactant P-20, 3.4mM EDTA, 10mM Hepes | 298 | 7.5 | 9609690 (Table II, p. 7985) | 1.25E-08 | -10.78 | |
| 217 | 1DVF | AB:CD | Mouse anti-hen egg white lysozyme monoclonal antibody D1.3 | Mouse antibody E5.2 | Ag-Ab | Surface Plasmon Resonance | 150mM NaCl, 0.005% Surfactant P-20, 10mM Hepes | 298 | 7.5 | 8703938 (Table 1, p. 9672) | 1.08E-08 | -10.86 | |
| 218 | 1DVF | AB:CD | Mouse anti-hen egg white lysozyme monoclonal antibody D1.3 | Mouse antibody E5.2 | Ag-Ab | Surface Plasmon Resonance | 150mM NaCl, 0.005% Surfactant P-20, 3.4mM EDTA, 10mM Hepes | 298 | 7.5 | 8993317 (Table 1, p. 52) | 1.08E-08 | -10.86 | |
| 219 | 3HFM | Y:LH | Mouse anti-lysozyme HyHEL-10 antibody IgG1 Fab | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | monitoring the rate of recovery of active HEWL | 298 | 7 | 10338006 (Appendix, pp. 966-7) | 2.87E-11 | -14.37 | ||
| 220 | 3HFM | Y:LH | Mouse anti-lysozyme HyHEL-10 antibody IgG1 Fab | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | Isothermal Titration Calorimetry | 50mM phosphate buffer with 0.2 M NaCl | 303 | 7.2 | 7629185 (Table III, p.18555) | 2.38E-09 | -11.96 | |
| 221 | 3HFM | Y:LH | Mouse anti-lysozyme HyHEL-10 antibody IgG1 Fab | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | Competitive Inhibition Enzyme Assay | 0.1% BSA, 66mM potassium phosphate | 298 | 6.24 | 7683415 (Table 1, p.3960) | 1.30E-10 | -13.48 | |
| 222 | 3HFM | Y:LH | Mouse anti-lysozyme HyHEL-10 antibody IgG1 Fab | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | Competitive Inhibition | 66mM potassium phosphate | 298 | 6.24 | 9761467 (Table I, p. 1859) | 7.50E-11 | -13.81 | |
| 223 | 3HFM | Y:LH | Mouse anti-lysozyme HyHEL-10 antibody IgG1 Fab | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | Competitive Inhibition | 66mM potassium phosphate | 298 | 6.24 | 9761468 (Table 1, p. 1870) | 7.00E-11 | -13.85 | |
| 224 | 1DQJ | AB:C | Mouse anti-lysozyme HyHEL-63 antibody IgG1 Fab | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | Surface Plasmon Resonance | 150mM NaCl, 0.005% Surfactant P-20, 10mM Hepes | 298 | 7.5 | 10828942 (Table 8, p. 6308) | 2.78E-09 | -11.67 | |
| 225 | 1DQJ | AB:C | Mouse anti-lysozyme HyHEL-63 antibody IgG1 Fab | Gallus gallus egg-white lysozyme C (HEL/HEWL) | Ag-Ab | Surface Plasmon Resonance | 150mM NaCl, 0.005% Surfactant P-20, 10mM Hepes | 298 | 7.5 | 12515535 (Table 2, p.15) | 2.78E-09 | -11.67 | |
| 226 | Mouse bone morphogenetic protein receptor type-1A (BMPR-1A) | Human recombinant bone morphogenetic protein 4 (BMP-4) | RC | Surface Plasmon Resonance | 10mM HEPES, 150mM NaCl, 3mM EDTA, 0.05% Tween20 | 298 | 7.4 | 11241215 (Table I, p. 403) | 4.64E-08 | -10 | |||
| 227 | 1AHW | AB:C | Mouse Ig 5G9 Fab | Human tissue factor/thromboplastin | Ag-Ab | Competition assay | 5mM CaCl2 | 298 | 9480775 (Table 4, p. 883) | 3.40E-09 | -11.55 | ||
| 228 | 1JRH | LH:I | Mouse Ig kappa chain C region (mAb A6) | Human interferon gamma receptor (IFN-γ-R1) | Ag-Ab | Surface Plasmon Resonance | 0.01 M HEPES, 0.15 M NaCl, 3mM EDTA, 0.005% v/v Surfactant P20 | 298 | 7.4 | 11123892 (Table I, p. 15676) | 1.20E-08 | -10.8 | |
| 229 | 1JRH | LH:I | Mouse Ig kappa chain C region (mAb A6) | Human interferon gamma receptor (IFN-γ-R1) | Ag-Ab | Surface Plasmon Resonance | 50mM sodium phosphate buffer, 150mM NaCl, 0.005% (v/v) Surfactant-p20 | 298 | 7.2 | 9367779 (Table 1, p. 885) | 1.42E-08 | -10.7 | |
| 230 | 1JRH | LH:I | Mouse Ig kappa chain C region (mAb A6) | Human interferon gamma receptor (IFN-γ-R1) | Ag-Ab | Surface Plasmon Resonance | 50mM sodium phosphate buffer, 150mM NaCl, 0.005 % (v/v) Surfactant p20 | 298 | 7.2 | 9878445 (Table 1, p. 808) | 1.44E-08 | -10.69 | |
| 231 | 1JRH | LH:I | Mouse Ig kappa chain C region (mAb A6) | Human interferon gamma receptor (IFN-γ-R1) | Ag-Ab | Competition ELISA | 50mM sodium phosphate buffer, 150mM NaCl, 0.005 % (v/v) Surfactant p20 | 298 | 7.2 | 9878445 (Table 3, p. 809) | 2.40E-09 | -11.75 | |
| 232 | Mouse interleukin-18 (mIL-18) | Human interleukin-18 binding protein (hIL-18BP) | Surface Plasmon Resonance | 0.01 M Hepes, 0.15 M NaCl, 3mM EDTA, 0.1% Tween 20 | 298 | 7.4 | 11278524 (Table 1, p. 17385) | 1.90E-11 | -14.62 | ||||
| 233 | Mouse interleukin-2 (mIL-2) | Human interleukin-2 receptor beta (hIL-2Rb) | Cold ligand competition assay | 200 µL HBSS, 0.1% BSA | 277 | 1600937 (Table I, p. 2048) | 3.00E-09 | -10.8 | IL receptor expressed in cos7 cells | ||||
| 234 | Mouse interleukin-2 (mIL-2) | Human interleukin-2 receptor beta (hIL-2Rb) | Cold ligand competition assay | 200 µL HBSS, 0.1% BSA | 277 | 1600937 (Table II, p. 2051) | 3.00E-10 | -12.07 | IL receptor expressed in BAF3 clones | ||||
| 235 | Mouse interleukin-2 (mIL-2) | Mouse high affinity interleukin-2 receptor (alpha/beta subunit, mIL-2R) | RC | Binding performed with 125I-labelled hIL-2 as 'hot' ligand; radioacitivity measured after separation of bound and free ligand | 100 µL HBSS, 0.1% BSA, 0.1% sodium azide | 310 | 1396584 (Table III, p. 3908) | 1.50E-11 | -15.35 | ||||
| 236 | Mouse interleukin-2 (mIL-2) | Mouse high affinity interleukin-2 receptor (alpha/beta subunit, mIL-2R) | RC | Binding performed with 125I-labelled hIL-2 as 'hot' ligand; radioacitivity measured after separation of bound and free ligand | 100 µL HBSS, 0.1% BSA, 0.1% sodium azide | 310 | 1396584 (Table III, p. 3908) | 6.00E-12 | -15.92 | ||||
| 237 | Mouse interleukin-2 (mIL-2) | Mouse high affinity interleukin-2 receptor (alpha/beta subunit, mIL-2R) | RC | Competitive Binding | 100 µL HBSS, 0.1% BSA, 0.1% sodium azide | 310 | 8262055 (Table II, p. 5115) | 2.50E-10 | -13.62 | ||||
| 238 | Mouse interleukin-2 (mIL-2) | Mouse low affinity interleukin-2 receptor (alpha subunit, mIL-2R) | RC | Competitive Binding | 100 µL HBSS, 0.1% BSA, 0.1% sodium azide | 310 | 8262055 (Table II, p. 5115) | 3.20E-09 | -12.05 | ||||
| 239 | 2JEL | P:HL | Mouse JEL42 antibody (Fab) | Escherichia coli phosphocarrier protein/histidine-containing protein (HPr) | Ag-Ab | Competition solid-phase radioimmune assay | PBS with 1% fetal calf serum | 298 | 1711212 (Table 1, p.4879); wild-type binding affinity value: 9671548 (Abstract, p. 765); | 3.70E-09 | |||
| 240 | 2O3B | A:B | Nostoc sp. nuclease (nucA) | Nostoc sp. sugar-non-specific nuclease inhibitor (nuiA) | EI | Measurement of plasmid DNA cleavage using agarose gel assay | 50mM Tris-HCl, 50mM NaCl, 1mM EDTA, 0.01% Triton X-100, 0.01% BSA, 5mM MnCl2 | 298 | 7 | 17138564 (Table 3, p. 5688) | 3.20E-12 | -15.67 | |
| 241 | 4E6K | A:G | Pseudomonas aeruginosa bacterioferritin/ferroxidase (bfrB) | Pseudomonas aeruginosa bacterioferritin-associated ferredoxin | OE | Surface Plasmon Resonance | PBS with 1.5 mM TCEP | 295 | 7.4 | 26368531 (Table 2, p. 6169) | 3.30E-06 | -7.4 | |
| 242 | 1F5R | A:I | Rattus norvegicus anionic trypsin-2 | Bovine pancreatic trypsin inhibitor (BPTI) | EI | Fluorescence | 100mM NaCl, 10mM CaCl2, and 50mM Hepes | 298 | 8 | 11420435 (Table 1, p. 1334) | 4.40E-10 | -12.76 | |
| 243 | Rattus norvegicus gap junction beta-1 protein/connexin-32 (Cx32) | Bovine calmodulin (CaM) | Isothermal Titration Calorimetry | 25mM Tris-HCl, 100mM KCl, 1mM β-ME, 5mM CaCl2 | 310 | 7 | 22718765 (Table 1, p. 27778) | 4.65E-06 | -7.56 | ||||
| 244 | 1LFD | A:B | Rattus norvegicus Ral guanine nucleotide dissociation stimulator (RalGDS) | Human GTPase HRas | GC | Stopped-flow Fluorescence | 15mM Hepes, 5mM MgCl2 | 298 | 7.4 | 15197281 (Table 2, supporting information) | 1.94E-06 | -7.79 | |
| 245 | Rattus norvegicus T-cell surface antigen CD2 | Rattus norvegicus CD48 antigen | Surface Plasmon Resonance | 150mM NaCl, 1mM CaCl2, 1mM MgCl2, 10mM Hepes, 0.005% Surfactant P-20 | 298 | 7.4 | 9576909 (Table 1, p. 5491) | 8.00E-05 | -5.59 | ||||
| 246 | 2LHI | A | Saccharomyces cerevisiae calmodulin (yCaM) | Saccharomyces cerevisiae calcineurin | Surface Plasmon Resonance | 20mM Tris/HCl with 1mM CaCl2, 250mM NaCl, 0.005% Tween 20 | 298 | 7.5 | 9756868 (Table II, p. 23679) | 5.70E-08 | -9.88 | ||
| 247 | 4NFG | A:B | Saccharomyces cerevisiae cytochrome c peroxidase, mitochondrial (CcP) | Equus caballus cytochrome c | OE | Isothermal Titration Calorimetry | 0.010 M potassium phosphate buffer, 0.050 M KNO3 | 298 | 6 | 9092837 (Table 1, p. 4056 and Table 2, p. 4059) | 1.00E-05 | -6.82 | |
| 248 | 2PCC | A:B | Saccharomyces cerevisiae cytochrome c peroxidase, mitochondrial (CcP) | Saccharomyces cerevisiae cytochrome c iso-1 | OE | Isothermal Titration Calorimetry | 50 mM Dimethylglutaric acid (DMG) | 298 | 6 | 11148036 (Table I, p. 423) | 1.15E-06 | -8.1 | |
| 249 | 2HRK | A:B | Saccharomyces cerevisiae glutamate-tRNA ligase, cytoplasmic | Saccharomyces cerevisiae tRNA-aminoacylation cofactor ARC1 | Surface Plasmon Resonance | 20mM Hepes, 150mM NaCl, 2mM EDTA, 0.005% Tween 20 | 298 | 7.4 | 17976650 (Table 2, p. 1083, Table 3, p. 1084) | 5.30E-08 | -9.92 | ||
| 250 | Serratia marcescens carbapenem-hydrolyzing beta-lactamase SME-1 | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | hydrolysis of cephalosporin C using spectrophotometry | 50 mm phosphate buffer with 1 mg/ml BSA | 298 | 7 | 12933802 (Table II, p. 45709) | 2.40E-09 | -11.75 | |||
| 251 | Serratia marcescens carbapenem-hydrolyzing beta-lactamase SME-1 | Streptomyces clavuligerus beta-lactamase inhibitor protein (BLIP) | EI | Spectroscopy | 50 mm phosphate buffer with 1 mg/ml BSA | 298 | 7 | 15284234 (Table II, p. 42862) | 2.40E-09 | -11.75 | |||
| 252 | 1FC2 | C:D | Staphylococcus aureus IgG-binding protein A | Human IgG1 MO61 Fc | Ag-Ab | 298 | 7716157 (Table II, p. 1468) | 1.50E-08 | -14.2 | ||||
| 253 | 1FC2 | C:D | Staphylococcus aureus IgG-binding protein A | Human IgG1 MO61 Fc | Ag-Ab | Competitive binding assay | PBS with 0.5% gelatin | 298 | 8332602 (Table II, p. 446) | 6.25E-08 | -14.2 | ||
| 254 | 1FC2 | C:D | Staphylococcus aureus IgG-binding protein A | Human IgG1 MO61 Fc | Ag-Ab | Surface Plasmon Resonance | 137 mM NaCl, 2.7 mM KCl, 1.5 mM KH2PO4, 8.1 mM Na2HPO4, 0.05% Surfactant P20 | 298 | 7.3 | 8588944 (Table 2, p. 275) | 1.68E-08 | -10.6 | |
| 255 | 1FCC | A:C | Streptococcus protein G domain B1 | Human IgG Fc fragment | Ag-Ab | Fluorescence | 20 mM phosphate buffer | 298 | 6 | 10452608 (Table 1, p. 1644) | 2.40E-07 | 0 | |
| 256 | 2NU0 | E:I | Streptomyces griseus streptogrisin-A/serine protease A (SGPA) | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Titration | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 9047374 (Table 1, p. 443) | 3.33E-12 | -15.44 | |
| 257 | 3SGB | E:I | Streptomyces griseus streptogrisin-B/serine protease B (SGPB) | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Spectroscopy | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 11171964 (data previously accessible online here, please check SKEMPI Notes for details on the data source) | 1.79E-11 | -14.46 | |
| 258 | 3SGB | E:I | Streptomyces griseus streptogrisin-B/serine protease B (SGPB) | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Spectroscopy | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 12767228 (Tables 1 and 2, p. 6462, Table 3, p. 6463) | 1.66E-11 | -14.5 | |
| 259 | 3SGB | E:I | Streptomyces griseus streptogrisin-B/serine protease B (SGPB) | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | 294 | Stephen Ming-teh Lu, PhD Thesis, Purdue University, 2000 (See SKEMPI for data source) | 1.79E-11 | -14.46 | ||||
| 260 | Sus scrofa chymotrypsin-like elastase family member 1, pancreatic/elastase-1 | Escherichia coli ecotin | EI | Fluorescence | 50mM Tris-HCl, 20mM CaCl2, 0.005% (w/v) Triton X-100 | 294 | 8.3 | 8143850 (Table 1, p.59) | 1.30E-09 | -11.95 | |||
| 261 | Sus scrofa chymotrypsin-like elastase family member 1, pancreatic/elastase-1 | Meleagris gallopavo (turkey) ovomucoid (OMTKY3) | EI | Titration | 0.1 M Tris-HCl, 0.005% Triton X-100, 0.02 M CaCl2 | 294 | 8.3 | 9047374 (Table 1, p. 443) | 2.38E-11 | -14.29 | |||
| 262 | 1FSS | A:B | Tetronarce californica acetylcholinesterase (AChE) | Dendroaspis angusticeps fasciculin-2 (fas-2) | EI | Enzyme activity assay using the colorimetric detection of AChE product | 50 mM phosphate buffer, 0.1 mg/ml BSA, 0.025% NaN3 (0.15 M) | 298 | 8 | 24613488 (Table 1, p. 639-40) | 3.70E-10 | -12.86 | |
| 263 | 3WWN | A:B | Thermus thermophilus LysW (TtLysW) | Thermus thermophilus LysZ (TtLysZ) | Isothermal Titration Calorimetry | 20mM HEPES-NaOH, 150mM NaCl | 333 | 8 | 25392000 (Table 2, p. 444) | 6.62E-06 | -7.89 | ||
| 264 | Xenopus laevis calmodulin | Rattus norvegicus nitric-oxide synthase, brain (NOS-1) | Isothermal Titration Calorimetry | 10 mM Hepes, 150 mM NaCl, 10 mM MgCl2, 3 mM CaCl2, 2 mM EGTA, 0.005% (v/v) Tween 20 | 298 | 7.4 | 12093276 (Table 1, p. 8601) | 6.45E-08 | -9.8 | ||||
| 265 | Xenopus laevis calmodulin | Rattus norvegicus nitric-oxide synthase, brain (NOS-1) | Fluorescence | 10 mM Hepes, 150 mM NaCl, 10 mM MgCl2, 3 mM CaCl2, 2 mM EGTA, 0.005% (v/v) Tween 20 | 298 | 7.4 | 12093276 (Table 2, p. 8602) | 6.15E-08 | -9.83 | ||||
| 266 | 1CF4 | A:B | Human cell division control protein 42 homolog (CDC42) | Human activated CDC42 kinase 1 (ACK-1) | GC | Isothermal Titration Calorimetry | 293 | 17999470 (Table 1, p. 14092) | 4.00E-08 | -9.92 |