Seq2Feature webserver is a comprehensive web-based feature extraction tool which computes protein and DNA sequence driven features. It can calculate 252 protein- based and 42 DNA- based descriptors. Major protein sequence based descriptors include physico-chemical, energetic and conformational properties, mutation matrices and contact potentials. These properties are widely used to predict protein structures and expression profiles. The DNA sequence based properties are physicochemical (Enthalpy, entropy, flexibility, stacking energy, melting point etc.), conformational (Bend, clash strength, rise, etc.) and composition based (GC content). Seq2Feature is a Python based toolkit to compute the numerical values associated to the DNA and protein sequences which can be useful for extracting the valuable descriptors for several prediction purposes.