Please Cite ProAffiMuSeq as

Jemimah S and Gromiha MM (2019). A sequence-based method to predict the binding free energy of protein-protein complexes upon mutation using functional classification. Manuscript submitted.

Thank You!

We gratefully acknowledge the various bioinformatics resources which we have used to develop the webserver. We have considered 103 amino acid properties (mainly derived from AAIndex) which were used to develop the Folding RaCe server. We also included 94 mutation matrices and 47 distance potentials from AAIndex. Further, we use the AACon standalone provided by the Barton group to calculate the conservation scores and PSI-BLAST to obtain the position-specific score matrix (PSSM).

• Folding RaCe
  Chaudhary P, Naganathan AN, Gromiha MM (2015). Folding RaCe: a robust method for predicting changes in protein folding rates upon point mutations. Bioinformatics. 31: 2091-2097.


• AAIndex
  Kawashima S et al (2008). AAindex: amino acid index database, progress report 2008. Nucleic Acids Res. 36: D202-D205.


• AACon
  Valdar WSJ (2002). Scoring residue conservation. Proteins: Struct, Funct, Genet. 48: 227-241.


• Manning JR, Jefferson ER and Barton GJ (2008). The contrasting properties of conservation and correlated phylogeny in protein functional residue prediction. BMC Bioinformatics. 9: 51.


• PSI-BLAST
  Altschul SF et al (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.