Zebrafish (Danio rerio) are highly fertile capable of producing hundreds of embryos, which are optically transparent and undergo rapid external development. Although Zebrafish hearts are similar in structural and histological aspects to humans, they have only two chambers and can completely regenerate upon injury. The vast genetic tools and rapid Zebrafish embryo development allows investigation of heart development, regeneration and gain insights to diseases. Here we outline the various microscopy strategies used to investigate intricate structural processes involved in the maturation of a beating linear heart tube into an efficient two-chamber pump. Some of the strategies used includes multi-view imaging using Lightsheet, reconstruction of four-dimensional beating heart by high-speed image acquisition across several planes (postprocessing) and real-time acquisition of three-dimensional images of beating hearts over developmental timescale. We show some of the early processes in cardiac development leading to chamber formation, looping, ballooning and trabeculation