Positionwise frequencies of all the amino acids

AA/Position	1	2	3	4	5	6
G	0.1406	0.0352	0.0273	0.0273	0.0859	0.0273
G	0.0156	0.0273	0.0117	0.0234	0.0039	0.0352
A	0.0273	0.0625	0.0195	0.0117	0.0195	0.0156
V	0.0234	0.0273	0.1445	0.0156	0.0273	0.0156
L	0.0234	0.0586	0.0352	0.1133	0.0273	0.0547
I	0.0234	0.0312	0.0234	0.1602	0.2422	0.0312
P	0.0156	0.0117	0.0156	0.0195	0.0195	0.0195
F	0.0391	0.0234	0.0898	0.0586	0.1094	0.0469
W	0.0117	0.0078	0.0273	0.0039	0.0078	0.0117
M	0.0117	0.0156	0.0078	0.0039	0.0039	0.0273
S	0.1562	0.0312	0.0312	0.0234	0.0117	0.0352
T	0.0312	0.1719	0.0156	0.0273	0.0078	0.0469
C	0.0000	0.0078	0.0156	0.0039	0.0000	0.0000
Y	0.0352	0.0430	0.0117	0.0312	0.0547	0.0156
Q	0.0117	0.0117	0.0234	0.0156	0.0156	0.0273
N	0.0312	0.0039	0.0469	0.0312	0.0195	0.0156
D	0.0273	0.0117	0.0234	0.0156	0.0195	0.0234
E	0.0469	0.0586	0.0625	0.0430	0.0234	0.1797
K	0.0703	0.0234	0.0273	0.0195	0.0234	0.0195
R	0.0312	0.0117	0.0117	0.0195	0.0078	0.0234
H	0.0195	0.0117	0.0078	0.0117	0.0078	0.0078

Average hydrophobicity = 36.27±10.14

Hydrophobicity values for 20 amino acids: A=13.26, C=26.84, D=8.15, E=11.12, F=90.17, G=3.80, H=4.14, I=69.53,

K=2.92, L=73.84, M=51.64, N=1.00, P=27.54, Q=6.00, R=10.24, S=3.53, T=11.64, V=44.60, W=100.00, Y=47.49

(Ref: Mant, CT et al. (2009). Biopolymers, 92, 6, 573-595.)

IITM	University of Madras